Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 818305

TRP channels overexpression contributes to inflammasome activation in clavicular cortical hyperostosis?


Lamot, Lovro; Vidović, Mandica; Perica, Marija; Bukovac Tambi, Lana; Gotovac, Kristina; Borovečki, Fran; Harjaček, Miroslav
TRP channels overexpression contributes to inflammasome activation in clavicular cortical hyperostosis? // Annals of the Rheumatic Diseases
Rim, Italija, 2015. str. 146-147 doi:10.1136/annrheumdis-2015-eular.2438 (predavanje, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 818305 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
TRP channels overexpression contributes to inflammasome activation in clavicular cortical hyperostosis?

Autori
Lamot, Lovro ; Vidović, Mandica ; Perica, Marija ; Bukovac Tambi, Lana ; Gotovac, Kristina ; Borovečki, Fran ; Harjaček, Miroslav

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Annals of the Rheumatic Diseases / - , 2015, 146-147

Skup
Annual European Congress of Rheumatology EULAR®

Mjesto i datum
Rim, Italija, 10-13.06.2015

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
chronic nonbacterial ; recurrent multifocal osteomyelitis ; CNO ; CRMO ; clavicular cortical hyperostosis ; CCH

Sažetak
Background: Clavicular cortical hyperostosis (CCH) is a sterile inflammatory bone disorder of unknown etiology clinically characterized by pain and/or swelling of the clavicle. It has been regarded as a variant of chronic nonbacterial/recurrent multifocal osteomyelitis (CNO/CRMO) but due to lack of other inflammatory sites and recurrence it could also be regarded as a separate disease in the spectrum [1]. Objectives: Identification of specific gene expression patterns in CCH patients. Methods: Total RNA was isolated from whole blood of 18 new-onset, untreated CCH patients and 8 healthy controls. DNA microarray gene expression was preformed in 5 CCH and 4 control patients along with bioinformatical analysis of retrieved data. Carefully selected differentially expressed genes (TRPM2, TRPM3, TRPM7, CASP2, MEFV, STAT3, EIF5A, ERBB2, TLR4, NLRP3, CD24, MYST3) where analyzed by qRT-PCR in all participants of the study. Results: Microarray results and bioinformatical analysis revealed 974 differentially expressed genes, while qRT-PCR analysis showed significantly higher expression of TRPM3 and TRPM7, and lower expression of ERBB2. Conclusions: Microarray data analysis reveled that majority of differentially expressed genes in CCH patients are involved in various inflammatory processes, while qRT-PCR analysis confirmed statistically significant expression change of 3 genes. Among them, TRPM3 and TRPM7 are members of transient receptor potential (TRP) gene superfamily, which encodes proteins that act as multimodal sensor cation channels for a wide variety of stimuli, one of which is environmental temperature that in the case of CCH could be elicited by overuse of sterno- clavicular joint (SCJ) [2]. Upon stimulation, TRP channels transduce electrical and/or Ca2+ signals. Dysfunctions in Ca2+ signaling due to altered TRP channel function can have strong effects on a variety of cellular and systemic processes, including the activation and the regulation of the inflammasomes, which are reported to be involved in CRMO pathogenesis [3, 4]. ERBB2, third gene with significant expression change, belongs to a family of genes that encodes for widely expressed cell surface growth factor receptors. Recently it has been shown that ErbB activation promotes protective cellular outcomes during inflammation, hence lower expression of this gene could cause damage due to inflammation [5]. Based on the results of these and previous studies, we hypothesize that CCH could be an autoinflammatory disease induced by SCJ overuse, TRP channel overexpression, inflammasome activation and reduced protection during inflammation. References: Borzutzky A, Stern S, Reiff A, et al. Pediatric chronic nonbacterial osteomyelitis. Pediatrics. 2012 Nov ; 130(5):e1190-1197. Shimizu S, Takahashi N, Mori Y. TRPs as chemosensors (ROS, RNS, RCS, gasotransmitters). Handbook of experimental pharmacology. 2014 ; 223:767-794. Latz E, Xiao TS, Stutz A. Activation and regulation of the inflammasomes. Nature reviews Immunology. 2013 Jun ; 13(6):397-411. Scianaro R, Insalaco A, Bracci Laudiero L, et al. Deregulation of the IL- 1beta axis in chronic recurrent multifocal osteomyelitis. Pediatric rheumatology online journal. 2014 ; 12:30. Frey MR, Brent Polk D. ErbB receptors and their growth factor ligands in pediatric intestinal inflammation. Pediatric research. 2014 Jan ; 75(1-2):127-132.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti



POVEZANOST RADA


Projekt / tema
108-1083107-0351 - Uloga biomarkera u patofiziologiji seronegativnih spondiloartropatija (Harjaček, Miroslav, MZOS - )

Ustanove
Medicinski fakultet, Zagreb,
KBC "Sestre Milosrdnice",
Dječja bolnica Srebrnjak

Citiraj ovu publikaciju

Lamot, Lovro; Vidović, Mandica; Perica, Marija; Bukovac Tambi, Lana; Gotovac, Kristina; Borovečki, Fran; Harjaček, Miroslav
TRP channels overexpression contributes to inflammasome activation in clavicular cortical hyperostosis? // Annals of the Rheumatic Diseases
Rim, Italija, 2015. str. 146-147 doi:10.1136/annrheumdis-2015-eular.2438 (predavanje, međunarodna recenzija, sažetak, znanstveni)
Lamot, L., Vidović, M., Perica, M., Bukovac Tambi, L., Gotovac, K., Borovečki, F. & Harjaček, M. (2015) TRP channels overexpression contributes to inflammasome activation in clavicular cortical hyperostosis?. U: Annals of the Rheumatic Diseases doi:10.1136/annrheumdis-2015-eular.2438.
@article{article, year = {2015}, pages = {146-147}, DOI = {10.1136/annrheumdis-2015-eular.2438}, keywords = {chronic nonbacterial, recurrent multifocal osteomyelitis, CNO, CRMO, clavicular cortical hyperostosis, CCH}, doi = {10.1136/annrheumdis-2015-eular.2438}, title = {TRP channels overexpression contributes to inflammasome activation in clavicular cortical hyperostosis?}, keyword = {chronic nonbacterial, recurrent multifocal osteomyelitis, CNO, CRMO, clavicular cortical hyperostosis, CCH}, publisherplace = {Rim, Italija} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati





    Contrast
    Increase Font
    Decrease Font
    Dyslexic Font