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Pregled bibliografske jedinice broj: 81780

Spatial and temporal traction response in human airway smooth muscle cells


Tolić-Norrelykke, Iva Marija; Butler, J. P.; Chen, J. X.; Wang, N.
Spatial and temporal traction response in human airway smooth muscle cells // American Journal of Physiology - Cell Physiology, 283 (2002), 4; C1254-C1266 (međunarodna recenzija, članak, znanstveni)


Naslov
Spatial and temporal traction response in human airway smooth muscle cells

Autori
Tolić-Norrelykke, Iva Marija ; Butler, J. P. ; Chen, J. X. ; Wang, N.

Izvornik
American Journal of Physiology - Cell Physiology (0363-6143) 283 (2002), 4; C1254-C1266

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Cell contraction; adhesion; mechanical stress; cytoskeleton; traction microscopy

Sažetak
Tractions that cells exert on their substrates are essential in cell spreading, migration, and contraction. These tractions can be determined by plating the cells on a flexible gel and measuring the deformation of the gel by using fluorescent beads embedded just below the surface of the gel. In this article we describe the image correlation method (ICM) optimized for determining the displacement field of the gel under a contracting cell. For the calculation of the traction field from the displacement field we use the recently developed method of Fourier transform traction cytometry (FTTC). The ICM and FTTC methods are applied to human airway smooth muscle cells during stimulation with the contractile agonist histamine or the relaxing agonist isoproterenol. The overall intensity of the cell contraction (the median traction magnitude, the energy transferred from the cell to the gel, and the net contractile moment) increased after activation with histamine, and decreased after treatment with isoproterenol. Cells exhibited regional differences in the time course of traction during the treatment. Both temporal evolution and magnitude of traction increase induced by histamine varied markedly among different cell protrusions, whereas the nuclear region showed the smallest response. These results suggest that intracellular mediators of cell adhesion and contraction respond to contractile stimuli with different rates and intensities in different regions of the cell. [References: 32]

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekt / tema
0098034

Ustanove
Institut "Ruđer Bošković", Zagreb

Autor s matičnim brojem:
Iva Marija Tolić, (260543)

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE