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Pregled bibliografske jedinice broj: 814710

Single-molecule imaging of cytoplasmic dynein in vivo


Ananthanarayanan, Vaishnavi; Tolić, Iva M.
Single-molecule imaging of cytoplasmic dynein in vivo // Methods in cell biology, 125 (2015), 1-12 doi:10.1016/bs.mcb.2014.10.001 (međunarodna recenzija, pregledni rad, znanstveni)


Naslov
Single-molecule imaging of cytoplasmic dynein in vivo

Autori
Ananthanarayanan, Vaishnavi ; Tolić, Iva M.

Izvornik
Methods in cell biology (0091-679X) 125 (2015); 1-12

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, pregledni rad, znanstveni

Ključne riječi
Diffusion ; Dynein ; Fission yeast ; HILO ; Live-cell imaging ; Microtubules ; Motor proteins ; Single-molecule observation ; TIRF ; Total internal reflection fluorescence microscopy

Sažetak
While early fluorescence microscopy experiments employing fluorescent probes afforded snapshots of the cell, the power of live-cell microscopy is required to understand complex dynamics in biological processes. The first successful cloning of green fluorescent protein in the 1990s paved the way for development of approaches that we now utilize for visualization in a living cell. In this chapter, we discuss a technique to observe fluorescently tagged single molecules in fission yeast. With a few simple modifications to the established total internal reflection fluorescence microscopy, cytoplasmic dynein molecules in the cytoplasm and on the microtubules can be visualized and their intracellular dynamics can be studied. We illustrate a technique to study motor behavior, which is not apparent in conventional ensemble studies of motors. In general, this technique can be employed to study single- molecule dynamics of fluorescently tagged proteins in the cell interior.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Biologija

Napomena
Biophysical Methods in Cell Biology ; ISBN: 9780128011034



POVEZANOST RADA


Ustanove
Institut "Ruđer Bošković", Zagreb

Autor s matičnim brojem:
Iva Marija Tolić, (260543)

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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