A Mild and Efficient Solid-Support Synthesis of Novel Oligonucleotide Conjugates (CROSBI ID 94470)
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Habuš, Ivan ; Xie, Jin ; Iyer, Radhakrishnan P. ; Zhou, Wen-Qiang ; Shen, Ling X. ; Agrawal, S.
engleski
A Mild and Efficient Solid-Support Synthesis of Novel Oligonucleotide Conjugates
Conjugates of oligodeoxyribonucleotide phosphorothioate (ODN-PS) with folic acid, retinoic acid, arachidonic acid, and methoxypoly(ethylene glycol)propionic acid have been synthesized. The procedure involved the initial solid-phase preparation of 5'-amino-functionalized ODN-PS using N-pent-4-enoyl-derived (PNT) nucleoside phosphoramidites followed by conjugation of the oligonucleotide either to the ligand acids, using 1-/3-(dimethylamino)propyl/-3-ethylcarbodiimide as a coupling reagent, or to their corresponding succinimidyl derivatives. Subsequent exposure of the support to aqueous ammonium hydrohide (28%, 2h, 55oC) resulted in the release of the fully deprotected ODN conjugates, which were purified by reversed-phase HPLC or by preparative polyacrylamide gel electrophoresis. The identity of the oligonucleotide conjugates was confirmed by MALDI-TOF mass spectral analysis.
Oligonucleotide conjugate synthesis
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