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Pregled bibliografske jedinice broj: 814031

Loss of p53 expression is accompanied with upregulation of beta-catenin in meningiomas: a concomitant reciprocal expression


Pećina-Šlaus, Nives; Kafka, Anja; Vladušić, Tomislav, Tomas, Davor; Logara, Monika; Skoko, Josip; Hrašćan, Reno
Loss of p53 expression is accompanied with upregulation of beta-catenin in meningiomas: a concomitant reciprocal expression // International journal of experimental pathology, 97 (2016), 2; 159-169 doi:10.1111/iep.12186 (međunarodna recenzija, članak, znanstveni)


Naslov
Loss of p53 expression is accompanied with upregulation of beta-catenin in meningiomas: a concomitant reciprocal expression

Autori
Pećina-Šlaus, Nives ; Kafka, Anja ; Vladušić, Tomislav, Tomas, Davor ; Logara, Monika ; Skoko, Josip ; Hrašćan, Reno

Izvornik
International journal of experimental pathology (0959-9673) 97 (2016), 2; 159-169

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Beta-catenin ; meningioma ; p53 ; protein expression ; Wnt and p53 signalling

Sažetak
A crosstalk between Wnt and p53 signalling pathways in cancer has long been suggested. We investigated the involvement of these pathways in meningioma by analysing their main effector molecules, beta- catenin and p53. Cellular expression of p53 and beta-catenin proteins and genetic changes in TP53 in 59 meningiomas were analysed by immunohistochemistry, PCR/RFLP and direct sequencing of TP53 exon 4. All findings were analysed with statistical analysis. Our analyses showed that 47.5% of the total sample demonstrated the loss of expression of p53 protein. Moderate and strong p53 expressions in the nucleus were observed, respectively, in 8.5% and 6.8% of meningiomas. Gross deletion of TP53 gene was observed in one meningioma (9% of samples), but nucleotide alterations were observed in 35.7% of meningiomas. Contrary, beta-catenin, the main Wnt signalling molecule, was upregulated in 71.2%, while strong expression was observed in 28.8% of meningiomas. The concomitant expressions of p53 and beta- catenin were investigated in the same patients. In the analysed meningiomas, the levels of the two proteins were significantly negatively correlated (P = 0.002). This indicates that meningiomas with lost p53 upregulate beta-catenin and activate Wnt signalling. Besides showing the reciprocal relationship between proteins, we also showed that the expression of p53 was significantly (P = 0.021) associated with higher meningioma grades (II and III), while beta-catenin upregulation was not associated with malignancy grades. Additionally, women exhibited significantly higher values of p53 loss when compared to males (P = 0.005). Our findings bring novel reports on p53 involvement in meningeal brain tumours and reveal the complex relationship between Wnt and p53 signalling, suggesting an important role of beta-catenin.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
HRZZ-IP-2013-11-6625 - Uloga signalnog puta Wnt u epitelno-mezenhimskoj tranziciji (Nives Pećina-Šlaus, )

Ustanove
Medicinski fakultet, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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