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Pregled bibliografske jedinice broj: 811722

Emergence of multidrug-resistant Proteus mirabilis in a long-term care facility in Croatia


Bedenić, Branka; Firis, Nataša; Elveđi- Gašparović, Vesna; Krilanović, Marija; Matanović, Krešimir; Štimac, Iva; Luxner, Josefa; Vraneš, Jasmina; Zarfel, Gernot; Grisold, Andrea
Emergence of multidrug-resistant Proteus mirabilis in a long-term care facility in Croatia // Wiener klinische Wochenschrift, 128 (2016), 11-12; 404-413 doi:10.1007/s00508-016-1005-x (međunarodna recenzija, članak, znanstveni)


Naslov
Emergence of multidrug-resistant Proteus mirabilis in a long-term care facility in Croatia

Autori
Bedenić, Branka ; Firis, Nataša ; Elveđi- Gašparović, Vesna ; Krilanović, Marija ; Matanović, Krešimir ; Štimac, Iva ; Luxner, Josefa ; Vraneš, Jasmina ; Zarfel, Gernot ; Grisold, Andrea

Izvornik
Wiener klinische Wochenschrift (0043-5325) 128 (2016), 11-12; 404-413

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
CMY-16 ; Proteus mirabilis ; Amp C β-lactamases ; conjugative plasmid ; clonal dissemination

Sažetak
SUMMARY Purpose: An increased frequency of Proteus mirabilis isolates resistant to expanded- spectrum cephalosporins was observed recently in a long-term care facility in Zagreb (Godan). The aim of this study was the molecular characterization of resistance mechanisms to new cephalosporins in P. mirabilis isolates from this nursing home. Methods: Thirty-eight isolates collected from 2013-2015 showing reduced susceptibility to ceftazidime were investigated. Antibiotic susceptibilities were determined by broth microdilution method. Inhibitor-based tests were performed to detect extended-spectrum (ESBLs) and AmpC β-lactamases. AmpC β- lactamases were characterized by PCR followed by sequencing of blaampC genes. Quinolone resistance determinants (qnr genes) were characterized by PCR. Genotyping of the isolates was performed by rep-PCR and PFGE (pulsed-field gel electrophoresis). Results:Presence of an AmpC β-lactamase was confirmed in all isolates by combined-disk test with phenylboronic acid. All isolates were resistant to amoxicillin alone and combined with clavulanate, cefotaxime, ceftriaxone, cefoxitin, and ciprofloxacin, but susceptible to cefepime, imipenem, and meropenem. PCR followed by sequencing using primers targeting blaampc genes revealed CMY-16 β-lactamase in all but one strain. Blacmy-16 was carried by a non-conjugative plasmid which did not belong to any known plasmid PCR-based incompatibility group (PBRT). Rep-PCR identified one large clone consisting of 15 isolates, three pairs or related isolates, one triplet and four singletons. PFGE confirmed the clonality of the isolates. Conclusions: This is the first report of multidrug resistant P. mirabilis in a nursing home in Croatia. Cephalosporin resistance was due to plasmid-mediated AmpC β- lactamase CMY- 16.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
108-1080114-0015 - Mehanizmi rezistencije na antibiotike u Gram-negativnih bakterija (Branka Bedenić, )
121-1080114-0306 - Djelovanje antibiotika na uzročnike biofilm infekcija (Jasmina Vraneš, )

Ustanove
Medicinski fakultet, Zagreb,
Nastavni zavod za javno zdravstvo "Dr. Andrija Štampar"

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • Index Medicus, Scopus


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