engleski

FORMATION OF ALKALI-SOLUBLE FLUORIDE ON THE SURFACE OF HUMAN DENTAL ENAMEL AFTER DIFFERENT TREATMENT TIME WITH AMINE FLUORIDE

Introduction: Topical application of high concentrations of fluoride (agents for professional application) onto dental enamel surface induces mainly formation of alkali-soluble fluoride described as a calcium fluoride or calcium fluoride-like material (CaF2). It is believed that calcium fluoride act as a pH-controlled fluoride-releasing reservoir. The released F ions to the solution phase surrounding the apatite crystals enhance remineralization and retard demineralization of enamel minerals. The various factors can influence on the formation of calcium fluoride on the teeth. The aim of this study was to quantify, in vitro, the formation of alkali-soluble fluoride after application an amine fluoride solution with different fluoride content and different treatment time, on the human dental enamel. Material and methods: Four enamel slabs were cut from each of 11 impacted third molars and randomly divided into four groups. One of them served as unfluoridated control (D). Only slabs without any visible defects were selected after examination under a magnifying glass (10x magnification). The slabs were mounted in dental wax, which covered all parts of the slabs except for the enamel surface (area approximately 15 mm2). Each of three groups of the slabs were shaked for 3 min in an amine fluoride solution of different fluoride concentrations with pH of 3.8 (Aminfluorid otopina â, Belupo, Croatia) ; group A: 1% F two times a day ; group B: 0.5% F three times a day and group C: 0.25% F four times a day. The treatments were repeated during two consecutive days. The formation of the alkali-soluble fluoride was analysed chemically by method of Caslavska et al. [1975]. The fluoride concentrations were determined by an ion selective electrode and the amount of alkali-soluble fluoride was calculated as described by Dijkman et al. [1983]. Results: The amount of alkali-soluble fluoride (ľg/cm2ąSD) was significantly higher in group C (115, 77 ą59.98) than in groups A (56.17ą48.95) or B (71.69ą37.03). No difference (p>0.05) was found between A and B groups (analysis variance Waller -Duncan's test). The amount of alkali-soluble fluoride on enamel increased with time of exposure despite of a lower content of fluoride. Conclusion: The result showed that increased frequency of fluoride applications even at a lower fluoride content could result in the formation of more calcium fluoride, what could be of clinical interest.

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