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A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real time quantitative PCR


White, Helen; Deprez, L; Corbisier, P; Hall, V; Lin, F; Mazoua, S; Trapmann, S; Aggerholm, A; Andrikovics, H; Akiki, S et al.
A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real time quantitative PCR // Leukemia, 29 (2015), 2; 369-376 doi:10.1038/leu.2014.217 (podatak o recenziji nije dostupan, članak, ostalo)


Naslov
A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real time quantitative PCR

Autori
White, Helen ; Deprez, L ; Corbisier, P ; Hall, V ; Lin, F ; Mazoua, S ; Trapmann, S ; Aggerholm, A ; Andrikovics, H ; Akiki, S ; Barbany, G ; Boeckx, N ; Bench, A ; Catherwood, M ; Cayuela, JM ; Chudleigh, S ; Clench, T ; Colomer, D ; Daraio, F ; Dulucq, S ; Farrugia, J ; Fletcher, L ; Foroni, Letizia ; Ganderton, R ; Gerrard G, Gineikienė E, Hayette S, El Housni, H ; Izzo, B ; Jansson, M ; Johnels P, Jurcek T, Kairisto V, Kizilors A, Kim DW, Lange T, Lion T, Polakova KM, Martinelli G, McCarron S, Merle, PA ; Milner B, Mitterbauer- Hohendanner, Gerlinde ; Nagar, M ; Nickless, G ; Nomdedéu, J ; Nymoen, DA ; Leibundgut, EO ; Ozbek, U ; Pajič, Tadej ; Pfeifer, H ; Preudhomme, C ; Raudsepp, K ; Romeo, G ; Sacha, T ; Talmaci, R ; Touloumenidou, T ; Van der Velden, VH ; Waits, P ; Wang, L ; Wilkinson, E ; Wilson, G ; Wren, D ; Zadro, Renata ; Ziermann, J ; Zoi, K ; Müller ; Martin ; Hochhaus, Andreas ; Schimmel, Heinz, Cross, Nicholas ; Emons, H.

Izvornik
Leukemia (0887-6924) 29 (2015), 2; 369-376

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, ostalo

Ključne riječi
Certified plasmid reference material ; bcr-abl1

Sažetak
Serial quantification of BCR–ABL1 mRNA is an important therapeutic indicator in chronic myeloid leukaemia, but there is a substantial variation in results reported by different laboratories. To improve comparability, an internationally accepted plasmid certified reference material (CRM) was developed according to ISO Guide 34:2009. Fragments of BCR– ABL1 (e14a2 mRNA fusion), BCR and GUSB transcripts were amplified and cloned into pUC18 to yield plasmid pIRMM0099. Six different linearised plasmid solutions were produced with the following copy number concentrations, assigned by digital PCR, and expanded uncertainties: 1.08 ± 0.13 × 106, 1.08 ± 0.11 × 105, 1.03 ± 0.10 × 104, 1.02 ± 0.09 × 103, 1.04 ± 0.10 × 102 and 10.0 ± 1.5 copies/μl. The certification of the material for the number of specific DNA fragments per plasmid, copy number concentration of the plasmid solutions and the assessment of inter-unit heterogeneity and stability were performed according to ISO Guide 35:2006. Two suitability studies performed by 63 BCR–ABL1 testing laboratories demonstrated that this set of 6 plasmid CRMs can help to standardise a number of measured transcripts of e14a2 BCR–ABL1 and three control genes (ABL1, BCR and GUSB). The set of six plasmid CRMs is distributed worldwide by the Institute for Reference Materials and Measurements (Belgium) and its authorised distributors (https://ec.europa. eu/jrc/en/reference- materials/catalogue/ ; CRM code ERM-AD623a-f).

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Ustanove
Farmaceutsko-biokemijski fakultet, Zagreb,
Klinički bolnički centar Zagreb

Citiraj ovu publikaciju

White, Helen; Deprez, L; Corbisier, P; Hall, V; Lin, F; Mazoua, S; Trapmann, S; Aggerholm, A; Andrikovics, H; Akiki, S et al.
A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real time quantitative PCR // Leukemia, 29 (2015), 2; 369-376 doi:10.1038/leu.2014.217 (podatak o recenziji nije dostupan, članak, ostalo)
White, H., Deprez, L., Corbisier, P., Hall, V., Lin, F., Mazoua, S., Trapmann, S., Aggerholm, A., Andrikovics, H. & Akiki, S. (2015) A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real time quantitative PCR. Leukemia, 29 (2), 369-376 doi:10.1038/leu.2014.217.
@article{article, year = {2015}, pages = {369-376}, DOI = {10.1038/leu.2014.217}, keywords = {certified plasmid reference material, bcr-abl1}, journal = {Leukemia}, doi = {10.1038/leu.2014.217}, volume = {29}, number = {2}, issn = {0887-6924}, title = {A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real time quantitative PCR}, keyword = {certified plasmid reference material, bcr-abl1} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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