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Balanced BMP - 9/TGF – β1 Ratio Safeguards Human Liver from Cirrhosis — Forensic Implications


Šoša, Ivan; Stemberga, Valter; Cuculić, Dražen
Balanced BMP - 9/TGF – β1 Ratio Safeguards Human Liver from Cirrhosis — Forensic Implications // 4th PANNONIA CONGRESS OF PATHOLOGY
Osijek, Hrvatska, 2016. (poster, sažetak, znanstveni)


Naslov
Balanced BMP - 9/TGF – β1 Ratio Safeguards Human Liver from Cirrhosis — Forensic Implications

Autori
Šoša, Ivan ; Stemberga, Valter ; Cuculić, Dražen

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
4th PANNONIA CONGRESS OF PATHOLOGY

Mjesto i datum
Osijek, Hrvatska, Od 12. do 15. svibnja 2016.

Vrsta sudjelovanja
Poster

Vrsta recenzije
Neobjavljeni rad

Ključne riječi
Bone morphogenetic protein-9; hepatocytes; liver regeneration; transforming growth factor – β1

Sažetak
Objective: Bone morphogenetic proteins (BMPs) are members of the TGF – β superfamily and play a role in places of epithelial - mesenchymal transition. Some BMPs exhibit the ability to regenerate organs. Since BMP-9 induces proliferation of human hepatoma cells and shows hypoglycemic effect like the one of insulin, it is possible that BMP - 9 helps regeneration of the damaged liver. Patients and Methods: We analysed, samples of human liver tissue. Human liver samples were obtained through autopsy from the Department of Forensic Medicine and Criminalistics, School of Medicine in Rijeka (N = 40). After pathohistological assessment, samples were further processed by immunohistochemistry, Western blot and gelatinase zymography. Results: In regard of the expression and localization of BMP - 9 and TGF – β1, we found them in the hepatocytes and bile duct epithelium of the healthy liver and in hepatocytes, bile duct epithelium and Kupfer cells of the cirrhotic liver. Quantitative analysis of the intensity of staining of both BMP-9 and TGF-β1 expressions showed statistically significant difference between the nodular and initial cirrhosis (P <0, 05) and between nodular cirrhosis and healthy liver (P<0, 05). Western blot analysis revealed the highest level of expression of BMP-9 and TGF-β1 in nodular cirrhosis. Significantly higher intensity of MMP - 2 expression was found in the nodular cirrhosis compared to healthy liver (P<0, 05). MMP-9 has significantly higher expression in the initial cirrhosis, compared to healthy liver (P<0, 05). Conclusion: The strongest intensity of both expressions of immunohistochemical stainings (BMP-9 and TGF-β1) was found in nodular cirrhosis. Western blot analysis confirmed immunohistochemistry findings, suggesting a fine balance between BMP-9 and TGF-β1 in the human liver to be pivotal for liver remodeling. Our findings of expression of the MMP- 2 and -9 confirmed the degree of damage of specimen, what could be useful in forensic practice.

Izvorni jezik
Engleski