Napredna pretraga

Pregled bibliografske jedinice broj: 810436

Emergence of OXA-48 beta-lactamase in Enterobacteriaceae in Croatia


Varda-Brkić, Dijana; Beader, Nataša; Čačić, Marko; Vranić-Ladavac, Mirna; Sardelić, Sanda; Jajić, Ines; Grisold, Andrea; Zarfel, Gernot; Luxner, Josefa; Bošnjak, Zrinka; Bedenić, Branka
Emergence of OXA-48 beta-lactamase in Enterobacteriaceae in Croatia // P0749
Amsterdam, Nizozemska, 2016. (poster, međunarodna recenzija, sažetak, znanstveni)


Naslov
Emergence of OXA-48 beta-lactamase in Enterobacteriaceae in Croatia

Autori
Varda-Brkić, Dijana ; Beader, Nataša ; Čačić, Marko ; Vranić-Ladavac, Mirna ; Sardelić, Sanda ; Jajić, Ines ; Grisold, Andrea ; Zarfel, Gernot ; Luxner, Josefa ; Bošnjak, Zrinka ; Bedenić, Branka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
P0749 / - , 2016

Skup
26th European congress of Clinical Microbiology and Infectious Diseases

Mjesto i datum
Amsterdam, Nizozemska, 09-12. 04. 2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
OXA-48; Klebsiella pneumoniae; resistance; carbapenems

Sažetak
Background : Previous studies found VIM-1, NDM- 1 and KPC-2 among CRE in Croatia with VIM-1 being the most prevalent. Recently OXA-48 was identified in three hospital centers. Material and methods: Since 2012 surveillance system for CRE was implemented in University Hospital Center (UHZ) in Croatia which inluded phenotypic and molecular identification of carbapenemases in Enterobacteriaceae. In total four carbapenem non-susceptible strains of K. pneumoniae were found to be positive for OXA-48 in three hospital centers (University Hospital Center Zagreb-UHZ, University Hospital Center Sisters of Mercy-UHM, University Hospital Split-UHS). The antimicrobial susceptibility was determined by broth microdilution method. Double-disk-synergy test (DDST) was performed to detect ESBLs and modified Hodge test (MHT) was used to screen for production of carbapenemases. MBL E-test was used to screen for metallo-β-lactamases (MBLs). Additionally the isolates were tested by combined disks test with EDTA or 3-aminophenylboronic to screen for KPC, MBLs or simoultaneous production of MBL and KPC respectively. The transferability of meropenem resistance was determined by conjugation employing E. coli A15R- strain resistant to sodium-azide. The presence of genes encoding broad and extended-spectrum β- lactamases (blaSHV, blaTEM, blaCTX-M and blaPER-1), plasmid-mediated AmpC β-lactamases, group A carbapenemases (blaKPC, blaSME, blaIMI, blaNDM), metallo β-lactamases (blaVIM, blaIMP and blaNDM), and carbapenem hydrolyzing oxacillinases (blaOXA-48), was determined by PCR. Results: The strains were uniformly resistant to amoxicillin alone and combined with clavulanate, cefazoline, cefuroxime, ceftazidime, cefotaxime, ceftriaxone, but uniformly susceptible to colistin and had variable resistance patterns to carbapenems. Modified Hodge test was positive indicating the production of carbapenemase. Phenotypic testing was positive for ESBL but negative for, MBL, KPC and AmpC. Meropenem resistance was not transferred to E. coli recipient strain. PCR revealed the strains to be positive also for CTX-M-15 and TEM-1. The strains from UHC coproduced OXA-48 and VIM-1. Two strains from UHZ had identical rep-PCR patterns but different from those from UHS and UHM. The patients did not have a travel history to the countries where OXA-48 is endemic. Conclusions The study demonstrated emergence of OXA-48 β- lactamase in three hospital centers located in different geographic regions in Croatia The strains from different hospitals displayed different rep_PCR patterns and thus it could be concluded that they occurred as independent events. OXA-48 was first report in Turkey in 2001 but later spread in Mediterranean and western European countries such as Israel, France, Italy, Spain, Germany, Switzerland, Belgium and the Netherlands. . Most of the strains in western Europe were imported from Turkey, Morocco, Egypt, Algeria or Lybia. Croatia was spared from this type of carbapenemas until 2014. In our study there was no link to the endemic areas.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
108-1080114-0015 - Mehanizmi rezistencije na antibiotike u Gram-negativnih bakterija (Branka Bedenić, )
121-1080114-0306 - Djelovanje antibiotika na uzročnike biofilm infekcija (Jasmina Vraneš, )

Ustanove
Medicinski fakultet, Zagreb,
Nastavni zavod za javno zdravstvo "Dr. Andrija Štampar"