engleski

Mitotic stability of small supernumerary marker chromosomes: a study based on 93 imortalized cell lines

Small supernumerary marker chromosome (sSMC) are known for being present in mosaic form as 47, +mar/45 in >50% of the cases with this kind of extra chromosomes. However, no detailed studies have been done for the mitotic stability of sSMC so far, mainly due to lack of corresponding in vitro model system.Recently, we established an sSMC-cell bank(Else-Kroner- Fresenius-sSMC-cellbank) with >150 cell lines. Therefore, 93 selected sSMC cases were studied here for the presence of the corresponding marker chromosomes before and after Epstein- Barr virus-induced immortalization. The obtained results showed that dicentric inverted duplicated-shaped sSMC are by far more stable in vitro than monocentric centric minute of ring-shaped sSMC. Simultaneously, a review of the literature revealed that a comparable shape-dependent mitotic stability can be found in vivo in sSMC carriers. Additionally, a possible impact of the age of the sSMC carrier on mitotic stability was found: sSMC cell lines established from patients between 10-20 years of age were predominantly mitotically unstable. The latter finding was indipendent of the sSMC shape. The present study shows that in vitro models can lead to new and exciting insights into the biology of the genetically and clinically heterogeneous patient group.

cell bank; cell lines; Epstein-Barr virus immortalization; marker chromosome shape; puberty; small supernumerary marker chromosome

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