engleski

Investigation of incorporation of amino acid into calcite using Mn2+ ions as paramagnetic probe

Calcite plays an important role in mineralized tissues of many organisms and is very important component in the natural environment as rocks and sediments. The calcitic skeletal elements of many organisms contain small amounts of proteins, which are incorporated within single crystals of calcite. For all of them the crystal size distribution and morphology of the crystals are of great significance and impurities of both inorganic and organic nature play here an important role. The aim of this study is to obtain initial information on the mechanisms of calcitic biominerals formation, in the simple model systems that could be relevant for the initial stages of mineralization. Several amino acids (Tyr, Asp, Lys) were used as models of soluble part of organic matrix that is supposed to be relevant for formation of specific calcium carbonate polymorph and morphology. In order to study changes of local environment of calcite crystal lattice regarding to incorporation of amino acids in calcite, by EPR spectroscopy, Mn2+ was added to systems as structural probe for EPR analysis, along with other analytical methods and technics (SEM, PXRD, FT-IR). The obtained results may shed light on the understanding of protein interaction with mineral phase.

biomineralizacija ; kalcit ; EPR ; aminokiseline

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