engleski

Impairment of neuronal cell cytoskeleton after RF irradiation

We evaluated impairment of polar and nonpolar, cytoskeleton filaments of neuronal cells, SH-SY5Y line, after modulated radiofrequency (RF) radiation. Cells were exposed to GSM modulated RF radiation frequency of 915 MHz. Applied electric field strength was 10, 20 and 30 V/m, and corresponding average specific absorption rate (SAR) of 0.23, 0.8 and 1.6 W/kg. Irradiation of the cells lasted for 1, 2, and 3 hours. Negative- and positive control samples were included. RF signal generator Gigahertz Transversal Electromagnetic Mode chamber (GTEM), RF power amplifier and RF signal modulator radiation were set-up for exposure treatment. The structure of microtubule and vimentin filaments were determined by means of immunofluorescence staining. To define actin microfilament structure, rhodamine-phalloidin fluorescent technique was used. Five hundred cells were examined under the fluorescent microscope to get insight into the structure of microtubule, actin and vimentin filaments. In comparison with negative control cell samples, microtubules and actin microfilaments were significantly impaired after three hour of exposure to 10, 20 and 30 V/m electric field strength. Opposing to microtubules and actin, the structure of vimentin filaments remained unchanged throughout the course of experiment. Impairment of microtubules and actin microfilaments was rather time then dose dependent. Related to the polar properties of filaments, cytoskeleton network can be damaged by RF radiation frequency of 915 MHz in time-dependent manner.

In vitro; RF/Microwaves;

nije evidentirano