engleski

Influence of Radiofrequency Radiation on Macromolecular Structure

Aim was to assess the biological activity of low-intensity, radiofrequency (RF) radiation on cell macromolecular structures ; DNA, and cytoskeleton fibres ; the microtubule and actin. Study was performed both in vivo by means of animal model and in vitro by using the cell culture. In vivo design included exposed rats (n=40) and unexposed controls (n=24). Both groups were divided in four subgroups. First exposed subgroup was irradiated with 2.45 GHz RF waves and an average power density of 7.5 mW cm-2, whole body SAR = 1.25 W kg-1, for 4 h in total, the second for 16h, the third for 30h, and the fourth for 60 h. Daily exposure in all groups was 2h a day. The animals were killed on exposure days 2, 8, 15, and 30. The in vitro design included continuous cell culture of V79 cells exposed to a 915 MHz RF field and 10 V/m-1 electric field, SAR=0.2 Wkg-1 for 1h, 2h, and 3h. Microtubule filaments were determined by immunofluorescence staining, and actin by rhodamine-phalloidin fluorescent technique. Micronucleus test (MN) on polychromatic erythrocytes (PCE) in vivo and V79 cells in vitro was performed. Significantly more PCE with MN in rats irradiated for 16h was found. In vitro study confirmed the significantly higher occurrence of micronucleated cells as well as damage to polar microtubules and actin filaments, which correlated with the duration of radiation exposure. In vivo and in vitro results confirm the undesired effects of man-made radiofrequency fields at the molecular level.

In vivo; RF/Microwaves;

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