engleski

Stamp gene as the highly discriminative marker for assessment of BN variability in Croatia

Since the specific membrane protein genes have been identified and characterized in ‘Candidatus Phytoplasma solani’genome, their variability has been extensively studied in order to elucidate different BN genotypes as well as the epidemiology of the disease. The aim of this study was to asses a variability of ‘Ca. P. solani’ strains from infected grapevine, wild plants and H.obsoletus by molecular characterization of stamp gene. Genotyping was performed on almost fifty BN strains detected in the scope of 4-year national survey (2009 – 2012) encompassing 277 samples from all viticultural regions of Croatia. Real-time PCR and conventional 16S rDNA were performed, followed by amplification, sequencing and phylogenetic analyses of stamp gene. The comprehensive analyses demonstrated the presence of ten stamp genotypes identified so far in Croatia, confirming the significance of this gene as a highly discriminative marker. The most prevalent genotype was identified (38%) being restricted to central north-western part of Croatia. Other two highly represented genotypes were also detected with one being present in central north-western and eastern part of Croatia (11%) and the other (9%) detected exclusively in coastal west part of Croatia. These results revealed considerable genetic variability, epidemiological relevance and the geographical distribution of stamp genotypes in Croatia.

Candidatus Phytoplasma solani; grapevine; MLST; stolbur

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