hrvatski

Ispitivanje procesiranja Scw4p proteina stanične stijenke u stanicama kex2 i 5ypsΔkex2 mutanata kvasca S. cerevisiae

Protein Scw4p is one of the most abundant non-covalently linked cell wall proteins in yeast and its role in the cell has not yet been discovered. It was previously shown that the protein is linked to the yeast cell wall by two different mechanisms - covalently and non-covalently. It is assumed that proteolytic enzymes which might have a role in processing of Scw4p are Kex2p and a family of aspartic proteases called yapsins and that non-covalently bound forms of Scw4p underwent the proteolytic processing, while the covalently bound Scw4p was not processed. Depending on the processing three forms of Scw4p in the cell wall with different molecular weights could be found. In this work Western blot analysis of Scw4p protein, isolated from 5ypsΔkex2 and kex2 mutant yeast transformed with native and mutated forms of the gene SCW4, was performed in order to get a better insight into the processing of Scw4p protein. Another object of this study was to determinate the impact of protein Scw4p overexpression on the viability of wild-type cells, kex2, 5ypsΔ and 5ypsΔkex2 mutants to gather information about the possible physiological role and possible differences in the activities of the various forms of this protein in the yeast cell wall.

Saccharomyces cerevisiae; stanična stijenka kvasca; protein Scw4p; Kex2p; japsinske proteaze

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