engleski

The effect of estrogen receptor genotypes on the number of stillborn and mummified piglets in Topigs 20 sows

Steroid hormones such as estrogen play a central role in postnatal female physiology and their effects are exerted through its receptors. The estrogen receptor gene is one of the first and most extensively investigated candidate genes for reproductive traits in pigs, especially for litter size. The aim of the study was to investigate the estrogen receptor gene polymorphism using endonuclease PvuII and its association with the number of stillborn (NSB) and number of mummified (NMUM) piglets in the first, second, third and subsequent parities and in overall parities. Topigs 20 sows (n=101) from 1st to 7th parities were analyzed. Estrogen receptor genotypes were detected by the polymerase chain reaction-restriction fragment length polymorphism method. Two alleles (A and B) were identified with three genotypes. Alleles (A and B) and genotype frequencies were determined. Comparison of observed and expected genotype frequencies was performed using the χ2-test and considerable deviation from Hardy-Weinberg principle was found. All data were analyzed using the General Linear Model. Statistical analysis showed a significant difference (P<0.05) in NMUM between the AB and BB genotypes in the third and subsequent parities. The statistical significance of differences between AA and BB genotypes tended to be lower (P<0.1) in the third and subsequent parities in NMUM. The sows with A allele had less NSB indicating a beneficial effect of this allele, unlike B allele in NMUM. The results obtained will contribute to the understanding of the effect of ESR genotype on NSB and NMUM and substantiate genetic evaluation of litter size traits in pigs.

estrogen; genotype; stillborn and mummified piglets; Topigs 20

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