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New perspectives of antitumor therapy - Silencing of heat shock proteins by siRNA nanodelivery

RNA interference (RNAi) is a mechanism of posttranscriptional regulation of gene expression. It can be triggered endogenously, by microRNA (miRNA), natural products of RNA polymerase II, or exogenously, by an uptake of an artificial double-stranded RNA, such as shRNA and siRNA. RNAi can be used as a potent therapeutic tool. The aim of this study was to examine a) the possibility of silencing the expression of the heat shock protein 70 (Hsp70), b) the possibility of delivery of siRNA for Hsp70 by chitosan nanoparticles and c) anti-tumor effect of Hsp70 silencing. RNAi was induced in Jurkat and U251N tumor cell lines by small siRNA for Hsp70 which was entrapped in chitosan nanoparticles. Chitosan nanoparticles showed low toxicity, efficient entrapping of Hsp70 siRNA and effective silencing of hsp70 gene in vitro. In Jurkat and U251N tumor cell lines silencing of the Hsp70 caused a decrease in viability of these tumor cell lines in two- and three-dimensional tumor models. RNA interference (RNAi), Heat shock proteins 70 (Hsp70), siRNA, chitosan nanoparticles

RNA interference (RNAi); Heat shock proteins 70 (Hsp70); siRNA; chitosan nanoparticles

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