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Applicability of three commercially available kits for forensic identification of blood stains (CROSBI ID 223628)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Horjan, Ivana ; Barbarić, Lucija ; Mršić, Gordan Applicability of three commercially available kits for forensic identification of blood stains // Journal of forensic and legal medicine, 38 (2016), 101-105. doi: 10.1016/j.jflm.2015.11.021

Podaci o odgovornosti

Horjan, Ivana ; Barbarić, Lucija ; Mršić, Gordan

engleski

Applicability of three commercially available kits for forensic identification of blood stains

Various commercially available one-step immunoassays for detection of human (primate) blood have been developed. This study evaluated two hemoglobin tests, ABAcard® HemaTrace® and HemDirect Hemoglobin against glycophorin A test-RSID™-Blood for following parameters: sensitivity, specificity, effectiveness using various substrates, stain remover and aged blood stains. The highest blood detection limit was observed if HemaTrace® was used. When compared with HemaTrace®, ten times lower sensitivity was observed for HemDirect Hemoglobin test. No false positives were obtained for HemDirect Hemoglobin while ABAcard® HemaTrace®, probably due to its extreme sensitivity, showed high percent of false positives with saliva. The lowest sensitivity and 40% of false positives with saliva was exhibited by RSID™-Blood. In addition, this test encountered the lowest efficacy if aged blood-stains or blood treated with stain remover were used. As expected, none of the tested substrates (wood, metal, brick, and soil), influenced on blood testing, although soil substrate affected STR amplification. Conducted studies established HemDirect Hemoglobin test as more reliable for evaluated parameters than ABAcard® HemaTrace® and RSID™-Blood.

forensic science; blood tests; sensitivity; specificity

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Podaci o izdanju

38

2016.

101-105

objavljeno

1752-928X

10.1016/j.jflm.2015.11.021

Povezanost rada

Biologija

Poveznice
Indeksiranost