engleski

Structural changes on bone a in male rat offspring depending on maternal diet

Introduction: Obesity as an epidemic problem induces many diseases including the metabolic syndrome. It causes low-grade systemic inflammation, which can also affect bone architecture. Proinflammatory cytokines are key mediators in osteoclast differentiation and bone resorption through the RANKL/RANK/OPG pathway. In our study we compared bone microstructure and TNF-α bone expression in animal subjects in dependence to diet exposed to in utero, during and after lactation. Methods: Ten female Sprague Dawley rats, nine weeks old, were randomly divided in two groups and fed either a control diet or food rich in saturated fatty acids during five weeks, and then mated with genetically similar male subjects. After birth and lactation male offspring rats from both groups were randomly divided in four groups depending on the diet they were fed until twenty-two weeks of age. Body weight throughout the experiment was measured and body mass index was calculated, total cholesterol, triglyceride level, IL-6 and TNF-α in serum were measured. Bone microstructure was analyzed in fifth lumbar vertebra (n=24) using digital photographic images. TNF-α expression was measured in bone samples using immunohistochemistry. Results: The offspring of healthy female rats fed with high content of saturated fatty acid food had the highest serum concentrations of the IL-6(157.39±48.38 pg/ml) and TNF-α (63.49±3.43 pg/ml) and also the highest intensity of the TNF-α immunohistochemical staining in the bone marrow. The same group had the lowest values of trabecular bone volume and trabecular number, but the highest values of trabecular separation. Conclusion:Such result suggests that saturated fatty acid food stimulates TNF-α expression in the bone marrow, which contributes to deterioration of the bone mass and bone structure in rat’s offspring

obesity; TNF-α; bone microstructure

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