engleski

Phytochemical characterization of polyphenols from endemic species Centaurea ragusina L. (Asteraceae)

Cultivation of medicinal plants on sparsely populated Adriatic islands is one potential initiator of their economic and social development. In order to help to choose the potential herbal species for cultivation, the scientific investigations of plants growing in Mediterranean areas are carried out with enhanced intensity. In this work, phytochemical characterization of a Croatian endemic species Centaurea ragusina L. (Asteraceae) was conducted. The presence of polyphenols was proven by using general reactions of developing colored products and precipitates. The thin layer chromatography revealed the presence of quercetin, rutin, and chlorogenic acid in the methanolic extract. Quantitative analysis of total polyphenols (TP), tannins (T), flavonoids (F), and phenolic acids (PA) was carried out using the spectrophotometric methods. The contents of analyzed polyphenolic compounds were as follows: 3.04±0.04% (TP), 0.36±0.04% (T), 0.25±0.00% (F), and 0.38±0.01% (PA). Conducted phytochemical characterization is a contribution to the scientific study of Croatian endemic species and completes the existing knowledge about phytotherapeutic potential of C. ragusina, especially in relation to the content of bioactive polyphenolic substances.

Asteraceae; Centaurea ragusina; polyphenols

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