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Diminished resistance to hyperoxia in brains of reproductively senescent female CBA/H mice (CROSBI ID 223131)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Šarić, Ana ; Sobočanec, Sandra ; Mačak Šafranko, Željka ; Popović Hadžija, Marijana ; Bagarić, Robert ; Farkaš, Vladimir ; Švarc, Alfred ; Marotti, Tatjana ; Balog, Tihomir Diminished resistance to hyperoxia in brains of reproductively senescent female CBA/H mice // Medical science monitor basic research, 21 (2015), 191-199. doi: 10.12659/MSMBR.895356

Podaci o odgovornosti

Šarić, Ana ; Sobočanec, Sandra ; Mačak Šafranko, Željka ; Popović Hadžija, Marijana ; Bagarić, Robert ; Farkaš, Vladimir ; Švarc, Alfred ; Marotti, Tatjana ; Balog, Tihomir

engleski

Diminished resistance to hyperoxia in brains of reproductively senescent female CBA/H mice

Background: We have explored sex differences in ability to maintain redox balance during acute oxidative stress in mice brain. We aimed to study if there were differences in oxidative/antioxidative status upon hyperoxia in brain of reproductively senescent CBA/H mice in order to elucidate some of the possible mechanisms of lifespan regulation. Material and Methods: The brains of 12 months old male and female CBA/H mice (n=9 per sex and treatment) subjected to 18h hyperoxia were evaluated for lipid peroxidation (LPO), antioxidative enzyme expression and activity - superoxide dismutase 1 and 2 (Sod-1, Sod-2), catalase (Cat), glutathione peroxidase 1 (Gpx- 1), heme- oxygenase 1 (Ho-1), nad NF-E2-related factor 2 (Nrf2), and for 2-deoxy-2-[18F] fluoro-D- glucose (18FDG) uptake. Results: No increase in LPO was observed after hyperoxia, regardless of sex. Expression of Nrf-2 showed significant downregulation in hyperoxia-treated males (p=0.001), and upregulation in hyperoxia- treated females (p=0.023). Also, in females hyperoxia upregulated Sod-1 (p=0.046), and Ho-1 (p=0.014) genes. SOD1 protein was upregulated in both sexes after hyperoxia (p=0.009 for males and p=0.011 for females). SOD2 protein was upregulated only in females (p=0.008) while CAT (p=0.026) and HO-1 (p=0.042) proteins were increased after hyperoxia only in males. Uptake of 18FDG was decreased after hyperoxia in the backbrain of females. Conclusion: We found that females at their reproductive senescence are more susceptible to hyperoxia, compared to males. We propose this model of hyperoxia as a useful tool to assess sex differences in adaptive response to acute stress conditions, which may be partially responsible for observed sex differences in longevity of CBA/H mice.

cell aging ; fluorodeoxyglucose F18 ; hyperoxia ; mice ; inbred CBA ; neuroimaging

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Podaci o izdanju

21

2015.

191-199

objavljeno

2325-4394

2325-4416

10.12659/MSMBR.895356

Povezanost rada

Temeljne medicinske znanosti

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