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Pregled bibliografske jedinice broj: 790088

Diminished resistance to hyperoxia in brains of reproductively senescent female CBA/H mice


Šarić, Ana; Sobočanec, Sandra; Mačak Šafranko, Željka; Popović Hadžija, Marijana; Bagarić, Robert; Farkaš, Vladimir; Švarc, Alfred; Marotti, Tatjana; Balog, Tihomir
Diminished resistance to hyperoxia in brains of reproductively senescent female CBA/H mice // Medical science monitor basic research, 21 (2015), 191-199 doi:10.12659/MSMBR.895356 (međunarodna recenzija, članak, znanstveni)


Naslov
Diminished resistance to hyperoxia in brains of reproductively senescent female CBA/H mice

Autori
Šarić, Ana ; Sobočanec, Sandra ; Mačak Šafranko, Željka ; Popović Hadžija, Marijana ; Bagarić, Robert ; Farkaš, Vladimir ; Švarc, Alfred ; Marotti, Tatjana ; Balog, Tihomir

Izvornik
Medical science monitor basic research (2325-4394) 21 (2015); 191-199

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Cell aging ; fluorodeoxyglucose F18 ; hyperoxia ; mice ; inbred CBA ; neuroimaging

Sažetak
Background: We have explored sex differences in ability to maintain redox balance during acute oxidative stress in mice brain. We aimed to study if there were differences in oxidative/antioxidative status upon hyperoxia in brain of reproductively senescent CBA/H mice in order to elucidate some of the possible mechanisms of lifespan regulation. Material and Methods: The brains of 12 months old male and female CBA/H mice (n=9 per sex and treatment) subjected to 18h hyperoxia were evaluated for lipid peroxidation (LPO), antioxidative enzyme expression and activity - superoxide dismutase 1 and 2 (Sod-1, Sod-2), catalase (Cat), glutathione peroxidase 1 (Gpx- 1), heme-oxygenase 1 (Ho-1), nad NF-E2-related factor 2 (Nrf2), and for 2-deoxy-2-[18F] fluoro-D-glucose (18FDG) uptake. Results: No increase in LPO was observed after hyperoxia, regardless of sex. Expression of Nrf-2 showed significant downregulation in hyperoxia-treated males (p=0.001), and upregulation in hyperoxia-treated females (p=0.023). Also, in females hyperoxia upregulated Sod-1 (p=0.046), and Ho-1 (p=0.014) genes. SOD1 protein was upregulated in both sexes after hyperoxia (p=0.009 for males and p=0.011 for females). SOD2 protein was upregulated only in females (p=0.008) while CAT (p=0.026) and HO-1 (p=0.042) proteins were increased after hyperoxia only in males. Uptake of 18FDG was decreased after hyperoxia in the backbrain of females. Conclusion: We found that females at their reproductive senescence are more susceptible to hyperoxia, compared to males. We propose this model of hyperoxia as a useful tool to assess sex differences in adaptive response to acute stress conditions, which may be partially responsible for observed sex differences in longevity of CBA/H mice.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
098-0982464-1647 - Sustav citokroma P450 i pojava tumora u starenju i oksidacijskom stresu (Tihomir Balog, )
098-0982464-2460 - Dobivanje struktura nalik Langerhansovim otočićima iz matičnih stanica miša (Mirko Hadžija, )
FP7-REGPOT-2012-2013-1
Grant Agreement Number 316289 – InnoMol

Ustanove
Institut "Ruđer Bošković", Zagreb

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Emerging Sources Citation Index (ESCI)
  • Scopus
  • MEDLINE


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