engleski

OPTIMIZATION OF METHODS FOR MOLECULAR SEX TYPING IN HERONS (ARDEIDAE)

Genetic markers are useful tool for tracking changes in population studies. This work, is focused on optimization of method for DNA isolation and molecular sex determination in herons (Ardeidae). Genomic DNA was isolated from feathers and eggshells by using two different protocols. For sex‐typing of herons we used primers 2550F/2781R for chromo‐helicase–DNA binding gene 1 (CHD1). Results showed that the highest DNA yield was obtained from the blood feathers and eggshells while mature feathers had the lowest yield. DNA yield was lower when commercial kit was used for isolation compared to the isolation with TNE buffer followed by addition of sodium acetate for keratin precipitation. Critical step is concentration of dithiothreitol (DTT) and proteinase K in lysis solution as well as the amount of blood present in the feathers.

genetic markers; sex‐typing; herons

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