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Determination of class III peroxidase and laccase isoforms during development of barley (Hordeum vulgare L.) stem (CROSBI ID 630480)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija

Begović, Lidija ; Pavoković, Dubravko ; Mlinarić, Selma ; Cesar, Vera Determination of class III peroxidase and laccase isoforms during development of barley (Hordeum vulgare L.) stem // Zbornik sažetaka 12. Hrvatskog biološkog kongresa s međunarodnim sudjelovanjem / Klobučar, Göran ; Kopjar, Nevenka ; Gligora Udovič, Marija et al. (ur.). Zagreb: Hrvatsko biološko društvo, 2015. str. 100-100

Podaci o odgovornosti

Begović, Lidija ; Pavoković, Dubravko ; Mlinarić, Selma ; Cesar, Vera

engleski

Determination of class III peroxidase and laccase isoforms during development of barley (Hordeum vulgare L.) stem

Class III peroxidases are involved in many physiological processes in plants. Together with laccases they are involved in oxidation of monolignols as the final step in the synthesis of lignin. Lignin is a natural polymer that builds the cell wall and provides mechanical strength to the stem. In the present study, objective was to determine the characteristics of individual isoforms of peroxidase and laccase in internodes of barley during the four developmental stages: elongation, heading, anthesis and grain filling. Total peroxidase (tPOD), laccase and peroxidase covalently bound to the cell wall (cPOD) were extracted and separated using isolelectric focusing on polyacrylamide gels. Guaiacol was used for detection of peroxidase activity and syringaldazine for laccase activity. The analysis showed the presence of eight different isoforms of tPOD with isoelectric point (pI) 8-10 and one isoform with pI 2.5-5. Highest activity was recorded in the first internode in all developmental stages. cPOD were present in a single isoform with activity profile similar to tPOD. Laccase were represented with two isoforms (pI 8-10 and pI 2.5-5) with no difference in activity in a particular internode and developmental stage. The results show the presence and characteristics of the various isoforms of peroxidase and laccase involved in development of barley stem. Future studies aim to clarify the role of these enzymes in the biosynthesis of lignin.

internode; cell wall; lignin; isoformes; multicopper oxidase

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Podaci o prilogu

100-100.

2015.

objavljeno

Podaci o matičnoj publikaciji

Zbornik sažetaka 12. Hrvatskog biološkog kongresa s međunarodnim sudjelovanjem

Klobučar, Göran ; Kopjar, Nevenka ; Gligora Udovič, Marija ; Lukša, Žaklin ; Jelić, Dušan

Zagreb: Hrvatsko biološko društvo

Podaci o skupu

"12. Hrvatski biološki kongres s međunarodnim sudjelovanjem"

predavanje

18.09.2015-23.09.2015

Sveti Martin na Muri, Hrvatska

Povezanost rada

Biologija