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Pregled bibliografske jedinice broj: 789010

Complexation between lysozyme and sodium poly(styrenesulfonate): the effect of pH, reactant concentration and titration direction


Štajner, Lara; Požar, Josip; Kovačević, Davor
Complexation between lysozyme and sodium poly(styrenesulfonate): the effect of pH, reactant concentration and titration direction // Colloids and surfaces. A, Physicochemical and engineering aspects, 483 (2015), 171-180 doi:10.1016/j.colsurfa.2015.03.034 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 789010 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Complexation between lysozyme and sodium poly(styrenesulfonate): the effect of pH, reactant concentration and titration direction

Autori
Štajner, Lara ; Požar, Josip ; Kovačević, Davor

Izvornik
Colloids and surfaces. A, Physicochemical and engineering aspects (0927-7757) 483 (2015); 171-180

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Polyelectrolytes ; Sodium poly(styrenesulfonate) ; Lysozyme ; Microcalorimetry ; Dynamic light scattering ; Zeta potential

Sažetak
It is well-known that mixing of aqueous solutions of oppositely charged proteins and polyelectrolytes leads to the formation of aggregates often called protein-polyelectrolyte complexes. In this study we investigated the interactions between aqueous solutions of lysozyme and sodium poly(styrenesulfonate) (PSS) as the model system for investigating protein-polyelectrolyte complexation processes. The experimental methods used in this study were isothermal titration microcalorimetry, electrophoretic mobility (i.e. zeta potential determination) and particle size (i.e. hydrodynamic radius) measurements. The effect of pH, reactant (protein and polyelectrolyte) concentration and titration direction (addition order) on lysozyme-PSS complexation was investigated at θ = 25 ºC and at ionic strength Ic = 10-2 mol dm-3. At all three examined pH values (pH = 3.1, 4.6 and 7.5) at low charge ratios a stable colloidal suspension was obtained in which charged colloid particles, whose sign of charge corresponds to that of the titrand, were present. On the other hand, at higher charge ratios large particles (floccules) appeared (with the hydrodynamic radius in the range 2 – 7 micrometers). These floccules were also charged and, at first, their charge sign was equal to that of the titrand (│zeta│ = 30 – 50 mV). However, upon further addition of the titrant the charge of the flocculae reversed as a result of the surplus of titrant molecules in the corona. The change in pH leads to the significant change in charge ratios at which the onset of flocculation is observed. In all examined cases the charge ratio at which zeta potential starts to change corresponds to the onset of flocculation determined by DLS. The reaction heat effects were determined and it was shown that in all examined cases complexation resulted in measurable negative enthalpy changes.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekti:
119-1191342-2961 - Fizikalna kemija koloida i međupovršina (Kallay, Nikola, MZOS ) ( POIROT)

Ustanove:
Prirodoslovno-matematički fakultet, Zagreb

Profili:

Avatar Url Josip Požar (autor)

Avatar Url Lara Jurković (autor)

Avatar Url Davor Kovačević (autor)

Citiraj ovu publikaciju

Štajner, Lara; Požar, Josip; Kovačević, Davor
Complexation between lysozyme and sodium poly(styrenesulfonate): the effect of pH, reactant concentration and titration direction // Colloids and surfaces. A, Physicochemical and engineering aspects, 483 (2015), 171-180 doi:10.1016/j.colsurfa.2015.03.034 (međunarodna recenzija, članak, znanstveni)
Štajner, L., Požar, J. & Kovačević, D. (2015) Complexation between lysozyme and sodium poly(styrenesulfonate): the effect of pH, reactant concentration and titration direction. Colloids and surfaces. A, Physicochemical and engineering aspects, 483, 171-180 doi:10.1016/j.colsurfa.2015.03.034.
@article{article, year = {2015}, pages = {171-180}, DOI = {10.1016/j.colsurfa.2015.03.034}, keywords = {Polyelectrolytes, Sodium poly(styrenesulfonate), Lysozyme, Microcalorimetry, Dynamic light scattering, Zeta potential}, journal = {Colloids and surfaces. A, Physicochemical and engineering aspects}, doi = {10.1016/j.colsurfa.2015.03.034}, volume = {483}, issn = {0927-7757}, title = {Complexation between lysozyme and sodium poly(styrenesulfonate): the effect of pH, reactant concentration and titration direction}, keyword = {Polyelectrolytes, Sodium poly(styrenesulfonate), Lysozyme, Microcalorimetry, Dynamic light scattering, Zeta potential} }
@article{article, year = {2015}, pages = {171-180}, DOI = {10.1016/j.colsurfa.2015.03.034}, keywords = {Polyelectrolytes, Sodium poly(styrenesulfonate), Lysozyme, Microcalorimetry, Dynamic light scattering, Zeta potential}, journal = {Colloids and surfaces. A, Physicochemical and engineering aspects}, doi = {10.1016/j.colsurfa.2015.03.034}, volume = {483}, issn = {0927-7757}, title = {Complexation between lysozyme and sodium poly(styrenesulfonate): the effect of pH, reactant concentration and titration direction}, keyword = {Polyelectrolytes, Sodium poly(styrenesulfonate), Lysozyme, Microcalorimetry, Dynamic light scattering, Zeta potential} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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