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Citrinin increases ochratoxin A-produced oxidative damage in kidney and liver of rats


Peraica, Maja; Rašić, Dubravka
Citrinin increases ochratoxin A-produced oxidative damage in kidney and liver of rats // Symposium "Power of fungi and mycotoxins in health and disease" : programme and abstracts
Šibenik, Hrvatska, 2015. str. 25-25 (poster, sažetak, znanstveni)


Naslov
Citrinin increases ochratoxin A-produced oxidative damage in kidney and liver of rats

Autori
Peraica, Maja ; Rašić, Dubravka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Symposium "Power of fungi and mycotoxins in health and disease" : programme and abstracts / - , 2015, 25-25

Skup
Symposium "Power of fungi and mycotoxins in health and disease"

Mjesto i datum
Šibenik, Hrvatska, 20.-23.9.2015.

Vrsta sudjelovanja
Poster

Vrsta recenzije
Neobjavljeni rad

Ključne riječi
Ochratoxin A; Citrinin; Oxidative stress; Kidney; Liver

Sažetak
Mycotoxins ochratoxin A (OTA) and citrinin (CTN) are fungal secondary metabolites often present together in food and feed. OTA is widely found mycotoxin and since its discovery it has been intensively studied. A number of OTA toxic effects are well known and target organs of its toxicity are kidney and liver. It is known that OTA induce oxidative damage in experimental animals. Although, OTA and CTN can co- occure in food and feed, data on CTN toxicity are rather scarce and the experiments are performed mostly on cell cultures. The purpose of our study was to check whether CTN treatment and combined treatment with CTN and OTA induce oxidative stress in kidney and liver of experimental animals. Adult male Wistar rats (N=6) were treated either with OTA (0.125 mg kg- 1 b.w., p.o., 21 d) or CTN (20 mg kg-1 b.w., p.o. 2 d) given separately or administered together. Spectrophotometer was used to measure glutathione (GSH) concentration while malondialdehyde (MDA) concentration was measured using HPLC with UV detector. Treatment with OTA alone significantly increased MDA concentration in kidney (145% compared with 100% of controls) which is in accordance with fact that kidney is primarily target of OTA toxicity. CTN is less toxic and treatment with CTN alone did not have significant effect on kidney and liver tissue. When animals were treated with both mycotoxins at the same time, MDA concentration in kidney and liver were significantly increased (194%, 160%, respectively) compared to controls. Treatment with either OTA or CTN did not significantly affect GSH concentration. When animals were treated with OTA and CTN at the same time, GSH was significantly increased in kidney and decreased in liver (70%) compared to controls (100%) which is in accordance with the fact that GSH is mostly synthetized in liver and then transported to other organs.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Ustanove
Farmaceutsko-biokemijski fakultet, Zagreb,
Institut za medicinska istraživanja i medicinu rada, Zagreb