Protective effects of resveratrol in ochratoxin A and citrinin-induced oxidative stress (CROSBI ID 630333)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Peraica, Maja ; Rašić, Dubravka ; Mladinić, Marin ; Želježić, Davor
engleski
Protective effects of resveratrol in ochratoxin A and citrinin-induced oxidative stress
Our previous studies have found that the nephrotoxic mycotoxin ochratoxin A (OTA) causes oxidative stress in rats and that this effect increases in combination with citrinin (CTN). Resveratrol (RSV) is a well-known antioxidative compound found in wine, grapes, dark chocolate and various berries. The purpose of this study was to establish whether RSV treatment could counteract oxidative stress in animals treated with OTA and CTN. Animals were treated orally in groups of 6 with OTA (0.125 and 0.250 mg kg−1 b.w.) with or without RSV (20 mg kg −1 b.w.) for 21 days. All of the animals were given CTN (20 mg kg−1 b.w.) orally on the last 2 days of OTA treatment. In the kidney and liver, the target organs of OTA and CTN toxicity, of both the treated animals and controls, glutathione (GSH) and malondialdehyde (MDA) concentrations were measured using spectrophotometry and HPLC, respectively. Oxidative DNA damage in kidney and liver homogenates was measured using the hOGG1 modified comet assay. RSV had a significant protective effect against oxidative stress caused by both doses of OTA (0.125 and 0.250 mg kg−1 b.w.) and CTN (20 mg kg −1 b.w.). The GSH concentration was higher in the kidney (0.304 ± 0.045, 0.256 ± 0.046 g g−1 tissue) of animals given both doses of OTA and CTN + RSV than that of animals given both OTA doses and CTN (0.200 ± 0.056, 0.164 ± 0.025 g g−1 tissue, p < 0.05). The same effect of RSV was seen in the liver regarding GSH (0.449 ± 0.081, 0.500 ± 0.100 g g−1 tissue, p < 0.05) compared to animals treated with OTA + CTN (0.300 ± 0.022, 0.298 ± 0.021 g g−1 tissue). RSV reduced MDA concentration in kidney of animals treated with OTA (0.250 mg kg−1 b.w.) + CTN + RSV (28.27 ± 3.33 ng g−1 tissue) compared to OTA + CTN treatment (42.48 ± 2.92) and in the liver of animals treated with OTA (0.125 mg kg−1) + CTN + RSV (11.1 ± 1.15) compared to controls (27.25 ± 2.90, p < 0.05). The tail intensity in the hOGG1 comet assay revealed RSV protection in the liver of animals treated with a lower dose of OTA + CTN, while DNA damage at the higher OTA dose + CTN remained significant (p < 0.05). RSV did not protect the kidney tissue from DNA oxidative damage from both OTA doses and CTN.
Resveratrol; ochratoxin A; citrinin; oxidative stress; kidney; liver
DOI: 10.1016/j.toxlet.2015.08.849
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Podaci o prilogu
S296-S296.
2015.
nije evidentirano
objavljeno
Podaci o matičnoj publikaciji
Toxicology letters
0378-4274
Podaci o skupu
Congress of the European Societies of Toxicology (61 ; 2015))
poster
13.09.2015-16.09.2015
Porto, Portugal
Povezanost rada
Temeljne medicinske znanosti