engleski

VERIFICATION OF QUANTITATIVE REAL-TIME (qPCR) microRNA PROFILING

MicroRNAs (miRNAs) derived from various tissues or organs have critical functions in many biological processes. miRNA profiling either in human serum or plasma could be clinically usefull in noninvasive molecular diagnostic testing, but its clinical effectiveness is likely to be affected by various methodological issues like the efficiency of RNA recovery and qPCR platform used. The aim of this study was verification of miRNAs profiling performance on TaqMan qPCR platform. Verification of qPCR miRNA profiling on TaqMan qPCR platform according to MIQE guidelines and manufacturers recommendations demonstrated good reproducibility which is a prerequisite for its clinical application.

microRNA; serum; plasma; MIQE; qPCR

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