engleski

Genotype by genotype dependence of hypovirus-induced stress in chestnut blight fungus Cryphonectria parasitica

Cryphonectria hypovirus 1 (CHV1), a virus conferring reduced virulence to chestnut blight fungus Cryphonectria parasitica, has been successfully used as a biocontrol agent. Several subtypes of the hypovirus have been isolated in Europe, having the impact on the fungus from mild to severe. It has been shown that hypovirus-infected fungal strains had a number of differentially expressed genes compared to the virus-free strains, among which were genes included in general stress response of the fungus. The objective of the study was to reveal whether the virus stress induction in fungus plays major role in decreasing fungal virulence. Various virus strains, previously characterised as mild or severe, were transferred to different Croatian isolates of C. parasitica and the activity of the stress enzymes catalase, glutathione S-transferase and superoxide dismutase was measured. Virus induced change in fungal stress enzymes activity, but the intensity and direction of the impact was dependent on virus strain and virus-host combination.

biocontrol ; chestnut blight ; hypovirulence

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