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Structural investigations of purine nucleoside phosphorylase from Helicobacter pylori I


Štefanić, Zoran; Leščić Ašler, Ivana; Mikleušević, Goran; Luić, Marija
Structural investigations of purine nucleoside phosphorylase from Helicobacter pylori I // 29th European Crystallographic Meeting Book of Abstracts
Zagreb, 2015. (poster, međunarodna recenzija, sažetak, znanstveni)


Naslov
Structural investigations of purine nucleoside phosphorylase from Helicobacter pylori I

Autori
Štefanić, Zoran ; Leščić Ašler, Ivana ; Mikleušević, Goran ; Luić, Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
29th European Crystallographic Meeting Book of Abstracts / - Zagreb, 2015

Skup
29th European Crystallographic Meeting

Mjesto i datum
Rovinj, Hrvatska, 23-28.08.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Purin nucleoside phosphorylase; Helicobacter pylori; dead-end complex

Sažetak
Helicobacter pylori is bacterial pathogen known for its ability to colonize and persist in human stomach (Makola et al., 2007). It is estimated that today H. pylori infects more than half of the world's population. Severe impact that H. pylori has on human health, makes the search for effective drugs to fight this pathogen of the utmost importance. Purine nucleoside phosphorylase (PNP) represents one promising drug target for this pathogen, as it is the key enzyme in the purine salvage pathway. H. pylori PNP is a homo- hexameric protein, and can be regarded as a trimer of dimers (Fig. 1a). The active site of each monomer can be in either open or closed conformation. Although the first solved crystal structure of very similar E. coli PNP, complexed with its ligands showed 3 open + 3 closed conformations (Koellner et al., 2002) we have recently determined several structures of E. coli PNP with 4 open + 2 closed sites conformation (Mikleušević et al., 2011). Two crystal structures of the PNP from the clinical isolate of H. pylori have been determined. Both belong to the orthorhombic crystal system and space group P212121. The crystal cells are different though: unit cell axes are 74, 129, 155 A (crystal grown at pH 7) and 104, 120, 139 A (crystal grown at pH 4.5). Interestingly, it was found in both structures, that the protein has 5 open + 1 closed conformations, which is the first such case, to the best of our knowledge. In the structure from pH 7 crystallization conditions, the closed active site is occupied with one phosphate ion (substrate) and one hypoxanthine molecule (product) in the so-called dead-end complex, while all five open sites are empty. The structure at pH 4.5 has the closed active site fully occupied (Fig. 1b), while the open sites are partially occupied with hypoxanthine and phosphate. Differences in active site conformations between H. pylori and E. coli PNP structures will be discussed as well as possible implications on the PNP mechanism of action.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekt / tema
HRZZ-IP-2013-11-7423 - Enzimi purinskog reciklirajućeg ciklusa iz Helicobacter pylori i Escherichie coli (Marija Luić, )

Ustanove
Institut "Ruđer Bošković", Zagreb