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Pregled bibliografske jedinice broj: 787438

In female rats, ethylene glycol treatment elevates protein expression of hepatic and renal oxalate transporter sat-1 (Slc26a1) without inducing hyperoxaluria


Breljak, Davorka; Brzica, Hrvoje; Vrhovac, Ivana; Micek, Vedran; Karaica, Dean; Ljubojević, Marija; Sekovanić, Ankica; Jurasović, Jasna; Rašić, Dubravka; Peraica, Maja et al.
In female rats, ethylene glycol treatment elevates protein expression of hepatic and renal oxalate transporter sat-1 (Slc26a1) without inducing hyperoxaluria // Croatian medical journal, 56 (2015), 447-459 doi:10.3325/cmj.2015.56.447 (međunarodna recenzija, članak, znanstveni)


Naslov
In female rats, ethylene glycol treatment elevates protein expression of hepatic and renal oxalate transporter sat-1 (Slc26a1) without inducing hyperoxaluria

Autori
Breljak, Davorka ; Brzica, Hrvoje ; Vrhovac, Ivana ; Micek, Vedran ; Karaica, Dean ; Ljubojević, Marija ; Sekovanić, Ankica ; Jurasović, Jasna ; Rašić, Dubravka ; Peraica, Maja ; Lovrić, Mila ; Schnedler, Nina ; Henjakovic, Maja ; Wegner, Waja ; Burckhardt, Gerhard ; Burckhardt, Birgitta C. ; Sabolić, Ivan ;

Izvornik
Croatian medical journal (0353-9504) 56 (2015); 447-459

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Gender differences ; immunocytochemistry ; kidney ; liver ; nephrolithiasis ; oxalemia ; oxaluria ; real time RT-PCR ; urolitiasis ; Western blotting

Sažetak
Aim To investigate whether the sex-dependent expression of hepatic and renal oxalate transporter sat-1 (Slc26a1) changes in a rat model of ethylene glycol (EG)-induced hyperoxaluria. Methods Rats were given tap water (12 males and 12 females ; controls) or EG (12 males and 12 females ; 0.75% v/v in tap water) for one month. Oxaluric state was confirmed by biochemical parameters in blood plasma, urine, and tissues. Expression of sat-1 and rate-limiting enzymes of oxalate synthesis, alcohol dehydrogenase 1 (Adh1) and hydroxy-acid oxidase 1 (Hao1), was determined by immunocytochemistry (protein) and/or real time reverse transcription polymerase chain reaction (mRNA). Results EG-treated males had significantly higher (in μmol/L ; mean ± standard deviation) plasma (59.7 ± 27.2 vs 12.9 ± 4.1, P < 0.001) and urine (3716 ± 1726 vs 241 ± 204, P < 0.001) oxalate levels, and more abundant oxalate crystaluria than controls, while the liver and kidney sat-1 protein and mRNA expression did not differ significantly between these groups. EG-treated females, in comparison with controls had significantly higher (in μmol/L) serum oxalate levels (18.8 ± 2.9 vs 11.6 ± 4.9, P < 0.001), unchanged urine oxalate levels, low oxalate crystaluria, and significantly higher expression (in relative fluorescence units) of the liver (1.59 ± 0.61 vs 0.56 ± 0.39, P = 0.006) and kidney (1.77 ± 0.42 vs 0.69 ± 0.27, P < 0.001) sat-1 protein, but not mRNA. The mRNA expression of Adh1 was female dominant and that of Hao1 male-dominant, but both were unaffected by EG treatment. Conclusions An increased expression of hepatic and renal oxalate transporting protein sat-1 in EG-treated female rats could protect from hyperoxaluria and oxalate urolithiasis.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
022-0222148-2142 - Toksični učinci mikotoksina na ljude i životinje (Maja Peraica, )

Ustanove
Institut za medicinska istraživanja i medicinu rada, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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