engleski

The efficiency of caffeic acid on oxidative stress and angiogenesis in Ehrlich ascites tumor

Progression of tumor cell development involves survival, proliferation, invasion, angiogenesis, and metastasis. Caffeic acid (CA) is an active component of propolis extract, which exhibits antioxidant, antiinflammatory, antiproliferative, cytostatic, antiangiogenic and most improtantly, antineoplastic properties. In the present study we investigated the effect of of caffeic acid on tumor growth and tumor angiogenesis in mice bearing Ehrlich ascites tumor (EAT). EAT cells (2.5x106) were implanted intraperitoneally (i.p.) in Swiss albino mice. After tumor inoculation, mice were injected i.p. with CA at dose of 40 and 80 mg kg‐1 bw in exponential growth phase from the 5 days after tumor cell injection (on day 5, 7, 9). On day 14, ascites volume, the total number of cells, differential count of the cells present in the peritoneal cavity, functional activity of macrophages, NO and antiangiogenic parameters were determined. The growth of EAT cells and formation of ascites in the peritoneum of EAT‐bearing mice was inhibited by CA. Further, results on decrease in the peritoneal angiogenesis and microvessel density show the antiangiogenic potential of CA in vivo. CA decreased NO level in tumor cells whereas NO level was increased in peritoneal macrophages. Taken together, we conclude that CA may increase the cytotoxic actions of macrophages by increasing NO and inhibit tumor growth and angiogenesis.

Ehrlich ascites tumor ; caffeic acid ; antiangiogenesis ; NO ; macrophages: M1 ; M2 and TAMs

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