engleski

Influence of solid-liquid extraction conditions on the proteins extraction from barley grains

Influence of extraction temperature (30-90 °C), concentration of ethanol/water solution (30-90%, v/v) and time (10-70 min) on extractability of proteins from barley grains was examined. Barley grain samples were collected from three different locations throughout Croatia: Nova Gradiška (sample BNG), Čakovec (sample BC) and Vinkovci (sample - BV). Box-Bhenken design of Response surface methodology (RSM) was used for identification of the best extraction conditions and extraction yield optimization. Total protein content (PC) in extracts was determined by the Bradford method, PCBR and Lowry method, PCLO. RSM analysis showed that ethanol concentration and extraction temperature had significant effect, while effect of time and interaction of factors on the observed extraction process were not significant. The optimal extraction conditions were achieved at 90 °C, 60%, v/v ethanol/water and 60 min with predicted maximum protein recovery, PCBR of 15.28 mgBSA/gdb, 16.74 mgBSA/gdb and 20.48 mgBSA/gdb for BNG, BC and BV, respectively. At the same extraction conditions, predicted maximum protein recovery, PCLO were 51.33 mgBSA/gdb, 57.64 mgBSA/gdb, and 56.90 mgBSA/gdb for BNG, BC and BV, respectively.

extraction; barley grains; protein recovery; optimization

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