engleski

The effect of Microwave Assisted Extraction (MAE) on the isolation of Polyphenols from Hawthorn

INTRODUCTION: Many studies have confirmed high biological potential of the extracts from hawthorn leaves and flowers, and beneficial effects to the human health. In recent years, the focus of many researches is the application of new extraction techniques for isolation of bioactive compounds from plant materials. The isolation of bioactive compounds from plant material by use of microwave assisted extraction (MAE) has shown great potential due to its numerous advantages compared with conventional extraction methods: lesser amount of solvent required, shorter extraction time, higher extraction rate and improved amount of bioactive compounds in extracts. OBJECTIVES: The objective of this study was to research the effect of MAE on the isolation of the total phenols (TPC) from hawthorn (Crataegi folium cum flore). The effect of microwave power, temperature of extraction, type and concentration of solvent (methanol, ethanol), and irradiation time were studied. METHODS: The extraction of TPC was performed using 30 and 50% aqueous solution of methanol and ethanol, at 50 and 65°C and microwave power from 200 to 400 W. The irradiation times were 3, 6, 9, 12 and 15 min. TPC were estimated spectrophotometrically using Folin Ciocalteu method and results were expressed as mg GAE /100g. RESULTS: Concentrations of solvent and irradiation time were the significant parameters for the extraction of total phenols (p≤0.01). Based on concentration of TPC, ethanol proved to be more effective solvents than methanol and amount of TPC increased till 6 minute of irradiation. Further increasing of irradiation time did not influence significantly on increasing of TPC. CONCLUSION: The results demonstrated that MAE could be a highly effective, reliable and fast method for extraction of phenolic compounds in hawthorn. The main advantage of MAE has been showed in higher concentration of total phenols extracted in shorter time compare to conventional extraction.

hawthorn ; microwave assisted extraction ; total phenols

nije evidentirano