engleski

Biocompatibility of maghemite nanoparticles with different surface coatings

The biomedical application of maghemite nanoparticles (-Fe2O3NPs) is intended for magnetic resonance and multimodal imaging, thermo-therapy, drug delivery, gene therapy. This study aimed to evaluate the biocompatibility of different -Fe2O3NPs by in vitro testing of their effects on oxidative stress response in neural stem cells (NSC) using staining methods with DCFH-DA, DHE and monochlorobimane molecular probes. The effect of uncoated (UNγ-Fe203NPs), poly-L-lysine-coated (PLLγ-Fe203NPs) and D-manose-coated (MANγ-Fe203NPs) maghemite NPs on intracellular reactive oxygen species (ROS) formation in NSC was evaluated. Significant decrease in ROS levels was obtained in NSC after treatment with MANγ-Fe203NPs and PLLγ-Fe203NPs as measured by the DHE assay, while significant decrease in ROS production was observed for UNγ-Fe203NPs from DCFH-DA staining. Exposure of NSC to all 3 types of γ-Fe203NPs resulted in dose-dependent reduction in the intracellular GSH levels. This indicates that all 3 types of γ-Fe203NPs initiated oxidative stress response in NSC. (Supported by EU FP7 grant GlowBrain: REGPOT-2012-CT2012-316120.)

superparamagentic iron oxide nanoparticles ; biocompatibility ; neural stem cells

The study was supported by EU FP7 grant GlowBrain (REGPOT-2012-CT2012-316120).