Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 782502

Polyelectrolyte Composite: Hyaluronic Acid Mixture with DNA


Marion, Ida Delač; Grgičin, Danijel; Salamon, Krešimir; Bernstorff, Sigrid; Vuletić, Tomislav
Polyelectrolyte Composite: Hyaluronic Acid Mixture with DNA // Macromolecules, 48 (2015), 8; 2686-2696 doi:10.1021/ma502090x (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 782502 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Polyelectrolyte Composite: Hyaluronic Acid Mixture with DNA

Autori
Marion, Ida Delač ; Grgičin, Danijel ; Salamon, Krešimir ; Bernstorff, Sigrid ; Vuletić, Tomislav

Izvornik
Macromolecules (0024-9297) 48 (2015), 8; 2686-2696

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DNA; SAXS; osmotic pressure; Manning condensation; liquid crystal; composites

Sažetak
We studied salt-free, highly concentrated (5–200 g/L) mixtures of unfragmented (μm contour length) DNA and hyaluronic acid (HA) as a borderline example of rigid-rod/flexible-chain composite, across a broad range of concentration ratios cHA/cDNA = 0.05–50. By polarizing microscopy, we established that the DNA and HA form clearly separated thread-like domains defined and oriented by solution shear. Within its domains, DNA shows birefringent banded patterns, routinely observed for long chain mesogens. We applied small-angle X-ray scattering (SAXS) to the mixtures and observed a polyelectrolyte (PE) correlation peak at q★ wave vector. This peak was ascribed to DNA subphase and was used as a measure of the effective DNA concentration in the subphase cDNA★, according to deGennes scaling relationship between the DNA mesh size ξ = 2π/q★∝ c–1/2 and monomer concentration c. From cDNA★ (and initial cHA and cDNA), we inferred the effective cHA★ of HA subphase. We find a concentration-independent ratio Γ = cHA★/ cDNA★ ≈ 0.85 across a broad range of 0.02–0.4 M. As there is the osmotic pressure (Π) equilibrium between DNA and HA subphases, the constant Γ indicates that Π ∝ c9/8 scaling common for DNA and other highly charged PEs is valid also for HA (a weak PE—does not feature counterion condensation). Therefore, we propose that this deviation from the conventional osmotic pressure scaling Π ∝ c cannot originate from the concentration dependence of counterion condensation, which is an implicit but common interpretation in the literature. Further, as HA releases all its counterions into the solution, the HA osmotic coefficient ϕHA we took as a measure of the DNA osmotic coefficient which we found to be ϕDNA ≈ 0.28. This, double the Manning value, corroborates a result by Raspaud et al.1

Izvorni jezik
Engleski

Znanstvena područja
Fizika



POVEZANOST RADA


Projekti:
UKF 17/13
035-0000000-2836 - Jako korelirani anorganski, organski i biomaterijali (Tomić, Silvia, MZOS ) ( POIROT)
035-0352843-2844 - Veza strukturnih i fizikalnih svojstava materijala kontrolirane dimenzionalnosti (Milat, Ognjen, MZOS ) ( POIROT)

Ustanove:
Institut za fiziku, Zagreb

Poveznice na cjeloviti tekst rada:

Pristup cjelovitom tekstu rada doi pubs.acs.org

Citiraj ovu publikaciju:

Marion, Ida Delač; Grgičin, Danijel; Salamon, Krešimir; Bernstorff, Sigrid; Vuletić, Tomislav
Polyelectrolyte Composite: Hyaluronic Acid Mixture with DNA // Macromolecules, 48 (2015), 8; 2686-2696 doi:10.1021/ma502090x (međunarodna recenzija, članak, znanstveni)
Marion, I., Grgičin, D., Salamon, K., Bernstorff, S. & Vuletić, T. (2015) Polyelectrolyte Composite: Hyaluronic Acid Mixture with DNA. Macromolecules, 48 (8), 2686-2696 doi:10.1021/ma502090x.
@article{article, author = {Marion, Ida Dela\v{c} and Grgi\v{c}in, Danijel and Salamon, Kre\v{s}imir and Bernstorff, Sigrid and Vuleti\'{c}, Tomislav}, year = {2015}, pages = {2686-2696}, DOI = {10.1021/ma502090x}, keywords = {DNA, SAXS, osmotic pressure, Manning condensation, liquid crystal, composites}, journal = {Macromolecules}, doi = {10.1021/ma502090x}, volume = {48}, number = {8}, issn = {0024-9297}, title = {Polyelectrolyte Composite: Hyaluronic Acid Mixture with DNA}, keyword = {DNA, SAXS, osmotic pressure, Manning condensation, liquid crystal, composites} }
@article{article, author = {Marion, Ida Dela\v{c} and Grgi\v{c}in, Danijel and Salamon, Kre\v{s}imir and Bernstorff, Sigrid and Vuleti\'{c}, Tomislav}, year = {2015}, pages = {2686-2696}, DOI = {10.1021/ma502090x}, keywords = {DNA, SAXS, osmotic pressure, Manning condensation, liquid crystal, composites}, journal = {Macromolecules}, doi = {10.1021/ma502090x}, volume = {48}, number = {8}, issn = {0024-9297}, title = {Polyelectrolyte Composite: Hyaluronic Acid Mixture with DNA}, keyword = {DNA, SAXS, osmotic pressure, Manning condensation, liquid crystal, composites} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • Nature Index


Citati:





    Contrast
    Increase Font
    Decrease Font
    Dyslexic Font