engleski

Cultivar Identity, Intravarietal Variation, and Health Status 3 of Native Grapevine Varieties in Croatia and Montenegro

Abstract: Croatia and Montenegro (southeast Europe) host numerous native (autochthonous) 22 minor grapevine varieties (Vitis vinifera L.) that might irreversibly disappear in the near future 23 due to limited conservation efforts and lack of virus-free propagation material. To prevent their 24 disappearance and help their sustainability, a collaborative project was launched to gather and 25 evaluate extremely rare and neglected native grapevine genotypes through establishment of true- 26 to-type and virus-tested stock material, “ready to use” for nurseries. Through several field 27 expeditions, 284 grapevine accessions that were collected in 22 different locations in Croatia and 28 Montenegro were subjected to ampelographic in situ description, genetic identification by nine 29 SSR markers, serological testing for the presence of common grape viruses, and intravarietal 30 variability assessment by S-SAP markers. Fifty-three unique SSR profiles were revealed, of which 25 genotypes were previously unpublished and thus worthy of further research. 32 Comparison of these genetic profiles to some very rare and poorly described accessions within 33 the European Vitis Database and other published studies revealed several unknown synonyms 34 and homonyms which helped properly name some profiles and provided novel information on 35 the history of these cultivars and their distribution in the region. Virus testing revealed high 36 frequency of infection with the GLRaV-3 and GLRaV-1 viruses and proved the urgent need for 37 sanitary selection. Assessment of intravarietal variation showed the presence of accumulated 38 mutations, which strongly suggests a need for clonal selection.

Vitis vinifera; SSR; S-SAP; ELISA; native varieties; identification; variability

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