engleski

Cryopreservation and cryotherapy of grapevine (Vitis vinifera L.)

This study aimed at establishing a cryopreservation protocol for grapevine shoot tips and at testing the efficiency of cryotherapy. In vitro cultures of healthy genotypes of eight Croatian autochthonous grapevine cultivars and of virus-infected genotypes of Plavac mali observed a high genotype effect and lower reactivity of infected genotypes. A PVS2-based cryopreservation protocol established led to approximately 50% recovery with cultivar Portan and three of the four international cultivars tested. By contrast, no or very low recovery was noted with the Croatian cultivars tested. ELISA tests revealed the elimination of the GFLV virus (77.8%) in cultivar Chardonnay and the GLRaV-3 virus (100%) in cultivar Cabernet Sauvignon in cryopreserved samples, respectively. Elimination was possible before cryopreservation. Immunolocalisation revealed GFLV-infected particles in meristematic tissues and GLRaV-3-infected particles in sieve elements. Genetic stability tested with eight AFLP primer combination, observed polymorphic fragments with increasing durations of exposure to PVS2 solution.

grapevine; cryopreservation; cryotherapy; genetic stability

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