Napredna pretraga

Pregled bibliografske jedinice broj: 779654

Investigation of patient samples quality control usability in assessment of solid immunoassay Investigation of patient samples quality control usability in assessment of solid immunoassay performance consistency: experience from six months foffow-up


Tešija Kuna, Andrea; Vukasović, Ines; Vrkić, Nada
Investigation of patient samples quality control usability in assessment of solid immunoassay Investigation of patient samples quality control usability in assessment of solid immunoassay performance consistency: experience from six months foffow-up // Autoantigens, Autoantibodies, Autoimmunity / Conrad, Karsten ; Chan, Edward K.L. ; Androde, Luis E.C. ; Steiner, Gilnter ; Pruijn, Ger J.M. ; Shoenfeld, Yehuda (ur.).
Dresden: PABST, 2015. str. 309-310 (poster, međunarodna recenzija, sažetak, znanstveni)


Naslov
Investigation of patient samples quality control usability in assessment of solid immunoassay Investigation of patient samples quality control usability in assessment of solid immunoassay performance consistency: experience from six months foffow-up

Autori
Tešija Kuna, Andrea ; Vukasović, Ines ; Vrkić, Nada

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Autoantigens, Autoantibodies, Autoimmunity / Conrad, Karsten ; Chan, Edward K.L. ; Androde, Luis E.C. ; Steiner, Gilnter ; Pruijn, Ger J.M. ; Shoenfeld, Yehuda - Dresden : PABST, 2015, 309-310

Skup
12th Dresden symposium on Autoantibodies

Mjesto i datum
Dresden, Njemačka, 23-26.09.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Autoantibodies; quality control; control samples

Sažetak
Use of manufacturer-independent quality control samples, especially clinical controls, has been encouraged for the long term assessment of autoimmune diagnostic test performance consistency. We aimed to compare the assessment of performance consistency for quantitative fluorescence enzyme immunoassays for detection of autoantibodies (EliA, Thermo Scientific) using manufacturer dependent (MQC) and clinical QC samples (CQC). In parallel we investigated the stability of CQC samples over time in conditions declared by the manufacturer. From January to June 2015 both positive and negative CQC were tested together with MQC in each run of measurement (EliA, Phadia 100, Thermo Scientific) of the following autoantibodies: anti-tissue transglutaminase IgA (anti-tTG IgA), anti-cyclic citrullinated IgG (anti-CCP), anti-cardiolipin IgG and IgM (aCL IgG, aCL IgM), anti-proteinase 3 IgG (anti-PR3) anti-myeloperoxidase IgG (anti- MPO) Our results showed acceptable precision for CQC as well as for MQC during the whole tested period for all respective antibodies. However, after four to five months, the positive CQC values tend downward for all except aCL IgM (tend upward). Use of CQC samples has advantages over MQC in terms of commutability, cut-off level precision assessment and potential ability for long-term follow-up, but also some disadvantages such as unknown stability or influence of endogenous substances.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti, Farmacija



POVEZANOST RADA


Ustanove
Farmaceutsko-biokemijski fakultet, Zagreb,
KBC "Sestre Milosrdnice"