engleski

Introduction of the three-tiered diagnostic system in Equine Infectious Anaemia surveillance in Croatia

The results of this pilot research proved that EIA in Croatia occurs with a low seroprevalence. This emphasizes the need of optimizing the serological diagnosis of the disease according to the epizootiological situation in order to confirm serologically positive animals, which are not numerous. This is an essential prerequisite for eradicating the disease. In order to improve the serological diagnosis, its effectiveness was tested by using the three-tiered approach in the epizootiological situation in Croatia. Because of the inadequate specificity and repeatability of the used ELISA kit, some questions aroused. The ELISA kit as the one used in this research would not give the advantage of speeding the process of making diagnosis and making it more accurate. Even more, using the ELISA kit with characteristics like this would make the diagnosis more expensive. Unlike the ELISA method, the IB method proved to be a necessary and functional additional diagnostic technique which ensures a more reliable, objective analysis. This research showed that the three-tiered approach, recommended by the World Organization for Animal Health, is not entirely appropriate to be used for EIA diagnosis without adjustments and validation. It seems that one of the crucial steps for obtaining the best results of the three-tiered system is to choose the adequate ELISA format. Based on these limited results, the modified GDP method has to remain the basis of EIA serological diagnosis, but introducing the IB method as a routine method for suspicious results is an objective possibility for improving the diagnosis, which can possibly lead to the eradication of the disease. To make final conclusions more sera samples should be tested using different ELISA kits.

EIA; three-tiered diagnostic system; surrvailence; Croatia

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