engleski

Comparison of gastrointestinal stability between Croatian wild edible plants: Case of phenolics

Qualitative and quantitative reversed-phase high performance liquid chromatography analyses of phenolics in water and ethanolic extracts of Croatian wild edible plants asparagus (Asparagus acutifolius L.), butcher’s broom (Ruscus aculeatus L.) and black bryony (Tamus communis L.) were conducted. Black bryony contained the highest amount of phenolics, followed by wild asparagus and butcher`s broom. Flavonol glycosides were the most abundant phenolics in black bryony and wild asparagus, in butcher`s broom phenolic acids were prevalent. Water extracts were subjected to two-phase (gastric and duodenal) in vitro digestion with human enzymes in order to compare the gastrointestinal stability of major phenolics in the selected plants extracts. Phenolic acids in all the tested water extracts were totally digested already after in vitro gastric digestion phase. Among flavonoids, two main kaempferol-glycosides from black bryony extract were best preserved (50% of each) after duodenal digestion. In wild asparagus extract approximately 20% of quercetin-3-O-rutinoside and isorhamnetin-3-O-rutinoside remained stable, and in the butcher`s broom extract no flavonoids could be detected upon duodenal digestion. Accordingly, among the tested plants black bryony would be the best source of natural antioxidants both because it contains the highest amount of flavonol glycosides, and because the highest percentage of this plant phenolics was preserved after in vitro gastrointestinal digestion.

Tamus communis ; Asparagus acutifolius ; Ruscus aculeatus ; flavonoids ; phenolic acids ; gastrointestinal stability ; energy content ; antioxidant activity

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