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New microsatellite markers for Campanula pyramidalis (Campanulaceae) and cross- amplification in closely related species

Microsatellite markers were identifi ed and characterized to study the genetic diversity and structure, conservation status, taxonomy, and biogeography of subspecifi c taxa and populations of Campanula pyramidalis (Campanulaceae). Eleven microsatellite markers were developed from genomic libraries enriched for di- and trinucleotide repeats. A total of 80 alleles were observed in the tested natural population. The number of alleles per locus, observed heterozygosity, and expected heterozygosity ranged from four to 13, 0.217 to 0.913, and 0.521 to 0.895, respectively. The new microsatellite markers will be useful for studying genetic diversity and structure as well as for better assessing the conservation status of subspecifi c taxa and populations of C. pyramidalis . Furthermore, a set of seven loci was successfully cross-amplifi ed in C. secundifl ora and C. versicolor and will be of great value for addressing unsolved taxonomic and biogeographic issues within the C. pyramidalis species complex.

Campanula pyramidalis; Campanulaceae; cross-amplification; microsatellites; simple sequence repeat (SSR)

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