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Pregled bibliografske jedinice broj: 776832

Pinpointing the interaction domains of plant seryl- tRNA synthetase and metabolic protein BEN1


Kekez, Mario; Zanki, Vladimir; Hodnik, Vesna; Anderluh, Gregor; Duchêne, Anne-Marie; Rokov Plavec, Jasmina
Pinpointing the interaction domains of plant seryl- tRNA synthetase and metabolic protein BEN1 // FEBS3+ Meeting: Molecules of Life / Kos, Janko ; Poklar Ulrih, Nataša (ur.).
Ljubljana: Slovenian Biochemical Society, 2015. str. 140-140 (poster, nije recenziran, sažetak, znanstveni)


CROSBI ID: 776832 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Pinpointing the interaction domains of plant seryl- tRNA synthetase and metabolic protein BEN1

Autori
Kekez, Mario ; Zanki, Vladimir ; Hodnik, Vesna ; Anderluh, Gregor ; Duchêne, Anne-Marie ; Rokov Plavec, Jasmina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
FEBS3+ Meeting: Molecules of Life / Kos, Janko ; Poklar Ulrih, Nataša - Ljubljana : Slovenian Biochemical Society, 2015, 140-140

ISBN
978-961-93879-0-0

Skup
FEBS3+: Molecules of Life

Mjesto i datum
Portorož, Slovenija, 16-19.09.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
protein-protein interaction ; BEN1 ; plant seryl-tRNA synthetase ; Aminoacyl-tRNA synthetases ; Brassinosteroids

Sažetak
Aminoacyl-tRNA synthetases (aaRS) support translational process by attaching appropriate amino acids to cognate tRNAs. Charged tRNA molecules reach ribosome and the amino acid can be transfered onto a growing peptide, according to the genetic code. It is well known that aaRSs can be involved in diverse cellular functions beyond translation independently or by forming protein- protein interactions. Understanding these non- canonical functions opens new perspectives in functional proteomics. However, the studies of plant aaRSs are scarce. Therefore our recent findings on interaction of BEN1, protein involved in metabolism of brassinosteroid hormones, and cytosolic seryl-tRNA synthetase (SerRS) from plant Arabidopsis thaliana broadens our knowledge of novel non-translational functions of plant aaRSs. We detected BEN1:SerRS interaction in vivo using Y2H screen and confirmed it in vitro using SPR method, also determining the dissociation constant of the protein complex. To pinpoint regions responsible for protein-protein interaction truncated variants of both SerRS and BEN1 protein were prepared and analyzed using microscale thermophoresis assay, that also enables Kd determination. Kd for complex containing BEN1 and truncated SerRS variant without basic C-terminal extension was similar to Kd of the SerRS:BEN1 complex indicating that basic C-terminal extension does not participate in interaction. Furthermore, N-terminal tRNA binding domain of SerRS did not form the complex with BEN1. Therefore we conclude that interaction involves the central part of SerRS that contains globular catalytic domain. Furthermore, using reverse transcription- quantitative polymerase chain reaction we investigated possible correlation of these two proteins on the level of gene expression in wild type plants, but also in transgenic plants overexpressing SerRS. We observed slightly lower expression of BEN1 gene in transgenic plants compared to wild type. Combining insights from both gene and protein level allowed us to define interaction surfaces and propose functional importance of SerRS:BEN1 assembly.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija



POVEZANOST RADA


Projekti:
MZOS-119-0982913-1358 - Strukturna raznolikost seril-tRNA sintetaza i točnost biosinteze proteina (Rokov Plavec, Jasmina; Weygand Đurašević, Ivana, MZOS ) ( POIROT)

Ustanove:
Prirodoslovno-matematički fakultet, Zagreb

Profili:

Avatar Url Vladimir Zanki (autor)

Avatar Url Jasmina Rokov Plavec (autor)

Avatar Url Mario Kekez (autor)


Citiraj ovu publikaciju:

Kekez, Mario; Zanki, Vladimir; Hodnik, Vesna; Anderluh, Gregor; Duchêne, Anne-Marie; Rokov Plavec, Jasmina
Pinpointing the interaction domains of plant seryl- tRNA synthetase and metabolic protein BEN1 // FEBS3+ Meeting: Molecules of Life / Kos, Janko ; Poklar Ulrih, Nataša (ur.).
Ljubljana: Slovenian Biochemical Society, 2015. str. 140-140 (poster, nije recenziran, sažetak, znanstveni)
Kekez, M., Zanki, V., Hodnik, V., Anderluh, G., Duchêne, A. & Rokov Plavec, J. (2015) Pinpointing the interaction domains of plant seryl- tRNA synthetase and metabolic protein BEN1. U: Kos, J. & Poklar Ulrih, N. (ur.)FEBS3+ Meeting: Molecules of Life.
@article{article, author = {Kekez, Mario and Zanki, Vladimir and Hodnik, Vesna and Anderluh, Gregor and Duch\^{e}ne, Anne-Marie and Rokov Plavec, Jasmina}, year = {2015}, pages = {140-140}, keywords = {protein-protein interaction, BEN1, plant seryl-tRNA synthetase, Aminoacyl-tRNA synthetases, Brassinosteroids}, isbn = {978-961-93879-0-0}, title = {Pinpointing the interaction domains of plant seryl- tRNA synthetase and metabolic protein BEN1}, keyword = {protein-protein interaction, BEN1, plant seryl-tRNA synthetase, Aminoacyl-tRNA synthetases, Brassinosteroids}, publisher = {Slovenian Biochemical Society}, publisherplace = {Portoro\v{z}, Slovenija} }
@article{article, author = {Kekez, Mario and Zanki, Vladimir and Hodnik, Vesna and Anderluh, Gregor and Duch\^{e}ne, Anne-Marie and Rokov Plavec, Jasmina}, year = {2015}, pages = {140-140}, keywords = {protein-protein interaction, BEN1, plant seryl-tRNA synthetase, Aminoacyl-tRNA synthetases, Brassinosteroids}, isbn = {978-961-93879-0-0}, title = {Pinpointing the interaction domains of plant seryl- tRNA synthetase and metabolic protein BEN1}, keyword = {protein-protein interaction, BEN1, plant seryl-tRNA synthetase, Aminoacyl-tRNA synthetases, Brassinosteroids}, publisher = {Slovenian Biochemical Society}, publisherplace = {Portoro\v{z}, Slovenija} }




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