engleski

Optimization of supercritical CO2 extraction of grape seed oil using response surface methodology

The aim of this study was to use waste from the wine production process with special accent on grape seeds and new green technology. Supercritical CO2 was considered as a green solvent in extraction of grape seed oil. The effects of different extraction process parameters on the oil yield and antioxidant activity were investigated. Extraction optimization was conducted using response surface methodology (RSM). Extraction pressure has proven to be the most significant factor influencing oil yield and antioxidation activity (p< 0.0001). The optimal conditions for obtaining the highest oil yield and antioxidant activity within the experimental range of the variables studied were at extraction pressure of 400 bar and temperature of 41 °C. Under these optimal conditions, the predicted extraction oil yield was 14.49% and DPPH 37.07%. Applying this green extraction method the oil from grape seeds was totally extracted. The produced oil was satisfactory quality and the content of α-tocopherol in obtained grape seed oil at optimal extraction conditions was 36.05 mg/kg.

grape seed oil; supercritical CO2 extraction; optimization; antioxidant activity

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