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Piscirickettsia salmonis infection in European sea bass – An emerging disease in Mediterranean mariculture (CROSBI ID 626980)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Zrnčić, Snježana ; Vendramin, Niccolò ; Boutrup, Torsten Snogdal ; Boye, Mette ; Bruun, Morten Sichlau ; Brnić, Dragan ; Oraić, Dražen Piscirickettsia salmonis infection in European sea bass – An emerging disease in Mediterranean mariculture // 17th International Conference on Diseases of Fish and Shellfish, Book of Abstracts / EAFP (ur.). Las Palmas: European Association of Fish Pathologists (EAFP), 2015. str. 153-153

Podaci o odgovornosti

Zrnčić, Snježana ; Vendramin, Niccolò ; Boutrup, Torsten Snogdal ; Boye, Mette ; Bruun, Morten Sichlau ; Brnić, Dragan ; Oraić, Dražen

engleski

Piscirickettsia salmonis infection in European sea bass – An emerging disease in Mediterranean mariculture

During late autumn 2013, two marine fish farms located in Croatia experienced disease outbreak leading to 20% mortality in the affected batch. Clinical disease appeared when water temperature was below16oC. Affected European Sea bass (D. labrax), weighting approximately 30 grams, showed abnormal swimming behavior, mainly characterized by whirling movements. Fish stop feeding and finally died few days after the appearance of clinical signs. Diseased specimens displayed pale livers, empty digestive tract with distended gall bladder ; the examination of the brain displayed hyperemia and congestion of the meninges. Bacteriological examination performed seeding heart, kidney and spleen on several bacteriological media incubated at 22oC during 48 hours did not show growth of any bacteria. Virological examination of brain homogenate, performed to rule out Nodavirus infection, using both susceptible cell culture SSN-1 and RT-qPCR tested negative. Histological examination of the brain tissue revealed multi focal areas of necrosis and extensive inflammation with predominantly histiocytic cells. Inflammatory cells displayed intra-cytoplasmatic basophilic vacuoles. The lumen of the brain ventricul appeared to be filled with cellular debris and abundant presence of eosinophilic macrophages was recorded. Based on these findings the suspicion of Piscirickettsia salmonis infection was formulated. Fluorescent in situ hybridization (FISH) using an oligonucleotide probe generically targeting the 16S rRNA region of bacteria tested positive once applied to fixed tissue sections of affected fish. The pathogen was identified by 16S rRNA gene sequencing using whole frozen brain material from affected fish and the sequence showed 99% similarity with P.salmonis. Finally, from the sequencing results a FISH probe for P.salmonis were designed and applied to histology section from affected fish, and this showed specific binding to the bacteria observed in the brain tissue sections. This pathogen has mainly been associated with disease in Atlantic salmon, and few reports in the Mediterranean basin are available. Further analysis will be attempted characterize it more precisely.

Piscirickettsia salmonis; European sea bass; Emerging disease; Croatia

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o prilogu

153-153.

2015.

objavljeno

Podaci o matičnoj publikaciji

17th International Conference on Diseases of Fish and Shellfish, Book of Abstracts

EAFP

Las Palmas: European Association of Fish Pathologists (EAFP)

Podaci o skupu

17th International Conference on Diseases of Fish and Shellfish

predavanje

07.09.2015-11.09.2015

Las Palmas de Gran Canaria, Španjolska

Povezanost rada

Veterinarska medicina