Napredna pretraga

Pregled bibliografske jedinice broj: 775810

Hormonal and epigenetic regulation during embryogenic tissue habituation in Cucurbita pepo L


Leljak-Levanić, Dunja; Mrvková, Mihaela; Turečková, Veronika; Pěnčík, Aleš; Rolčík, Jakub; Strnad, Miroslav; Mihaljević, Snježana
Hormonal and epigenetic regulation during embryogenic tissue habituation in Cucurbita pepo L // Plant cell reports, 35 (2016), 1; 77-89 doi:10.1007/s00299-015-1869-7 (međunarodna recenzija, članak, znanstveni)


Naslov
Hormonal and epigenetic regulation during embryogenic tissue habituation in Cucurbita pepo L

Autori
Leljak-Levanić, Dunja ; Mrvková, Mihaela ; Turečková, Veronika ; Pěnčík, Aleš ; Rolčík, Jakub ; Strnad, Miroslav ; Mihaljević, Snježana

Izvornik
Plant cell reports (0721-7714) 35 (2016), 1; 77-89

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
ABA ; CKs ; DNA methylation ; habituation ; IAA ; somatic embryogenesis

Sažetak
A comparative analysis between habituated and non- habituated embryogenic cultures of pumpkin (Cucurbita pepo L.) in relation to endogenous phytohormones, global DNA methylation, developmental and regeneration capacity of the cultures was conducted. The analysis revealed more cytokinins (CKs) and indole-3-acetic acid (IAA), but less abscisic acid (ABA) in the habituated HEC line than in the non-habituated DEC line. Ribosides and ribotides were the most abundant CK forms in both HEC and DEC lines (75.9 and 57.6% respectively). HEC contained more free-base CKs (5.8 vs. 3.2%) whereas DEC contained considerably more O-glycosides (39.1 vs. 18.3%). Though prevalence of IAA was common for both lines, relative ratio of CKs and ABA differed between DEC and HEC lines. ABA was prevailing over CKs in DEC, while CKs prevailed over ABA in HEC line. Taking into account the importance of ABA for embryo maturation, the reduced endogenous ABA content in HEC line might be the reason for a 5-fold reduction in regeneration capacity compared to DEC. Both habituated and non-habituated embryogenic lines were highly methylated in the presence of 2, 4- dichlorophenoxyacetic acid (2, 4- D). Pronounced hypomethylation correlated with the absence of 2, 4-D, addition of 5-azacytidine (5- azaC), but also with the process of habituation. The habituated line was resistant to the effect of hypomethylation drug 5-azaC and remained highly methylated even after the addition of 5-azaC. Also, 5-azaC did not change the developmental pattern in the habituated line, indicating the existence of separate mechanisms by which 2, 4-D influences global DNA methylation in comparison to habituation- related global DNA methylation.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekt / tema
098-0982913-2829 - Molekularna regulacija biljnog razvitka (Branka Salopek-Sondi, )
119-1191196-1225 - Faktori i biljezi embriogenog potencijala i fenolnog metabolizma u biljaka (Sibila Jelaska, )

Ustanove
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati