TROL-FNR interaction reveals alternative pathways of electron partitioning in photosynthesis (CROSBI ID 220963)
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Vojta, Lea ; Carić, Dejana ; Cesar, Vera ; Antunović Dunić, Jasenka ; Lepeduš, Hrvoje ; Kveder, Marina ; Fulgosi, Hrvoje
engleski
TROL-FNR interaction reveals alternative pathways of electron partitioning in photosynthesis
In photosynthesis, final electron transfer from ferredoxin to NADP+ is accomplished by the flavo enzyme ferredoxin:NADP+ oxidoreductase (FNR). FNR is recruited to thylakoid membranes via integral membrane thylakoid rhodanase-like protein TROL. We address the fate of electrons downstream of photosystem I when TROL is absent. We have employed electron paramagnetic resonance (EPR) spectroscopy to study free radical formation and electron partitioning in TROL-depleted chloroplasts. DMPO was used to detect superoxide anion (O2.−) formation, while the generation of other free radicals was monitored by Tiron. Chloroplasts from trol plants pre-acclimated to different light conditions consistently exhibited diminished O2.− accumulation. Generation of other radical forms was elevated in trol chloroplasts in all tested conditions, except for the plants pre-acclimated to high-light. Remarkably, dark- and growth light-acclimated trol chloroplasts were resilient to O2.− generation induced by methyl-viologen. We propose that the dynamic binding and release of FNR from TROL can control the flow of photosynthetic electrons prior to activation of the pseudo-cyclic electron transfer pathway.
photosynthetic electron transport ; Photosystem I ; ROS ; electron paramagnetic resonance
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