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izvor podataka: crosbi

Rapid transient expression of human granulocyte-macrophage colony-stimulating factor in two industrial cultivars of tobacco (Nicotiana tabacum L.) by agroinfiltration (CROSBI ID 220959)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Vojta, Lea ; Ljuma-Skupnjak, Lana ; Budimir, Ankica ; Vukičević, Slobodan ; Fulgosi, Hrvoje Rapid transient expression of human granulocyte-macrophage colony-stimulating factor in two industrial cultivars of tobacco (Nicotiana tabacum L.) by agroinfiltration // Biotechnology reports, 7 (2015), 81-86. doi: 10.1016/j.btre.2015.05.006

Podaci o odgovornosti

Vojta, Lea ; Ljuma-Skupnjak, Lana ; Budimir, Ankica ; Vukičević, Slobodan ; Fulgosi, Hrvoje

engleski

Rapid transient expression of human granulocyte-macrophage colony-stimulating factor in two industrial cultivars of tobacco (Nicotiana tabacum L.) by agroinfiltration

We report the production of hGM-CSF cytokine in leaves of industrial tobacco cultivars DH-17 and DH-27 by using Agrobacterium-mediated transient expression. We prove the concept that very high biomass industrial tobacco plants are suitable platforms for rapid, low cost production of foreign proteins. Successful transient expression of the GM-CSF was achieved in less than three months, opening the possibility for future applications of this approach in rapid response production of various proteins of non-plant origin in industrial tobacco.

tobacco; transient expression; GM-CSF; vacuum infiltration; heterologous expression

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Podaci o izdanju

7

2015.

81-86

objavljeno

2215-017X

10.1016/j.btre.2015.05.006

Povezanost rada

Biotehnologija, Biologija

Poveznice
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