Rapid and low cost production of hGM-CSF cytokine in industrial tobacco – proof of concept (CROSBI ID 626901)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Vojta, Lea ; Ljuma-Skupnjak, Lana ; Budimir Ankica ; Vukičević Slobodan ; Fulgosi, Hrvoje
engleski
Rapid and low cost production of hGM-CSF cytokine in industrial tobacco – proof of concept
We report the production of hGM-CSF protein in leaves of two industrial cultivars of tobacco by using Agrobacterium-mediated transient expression. For overexpression, two binary destination vectors, p2GW7.0 and pH7WG2.0, were evaluated. The gene hGM-CSF was tagged on the 3’ end with the His and HA-epitopes and cloned into the destination vectors. Agrobacteria containing constructs GM-CSFHis- HA/pH7WG2.0 and GM-CSF-His-HA/p2GW7.0 were used for 5- and 10-minute-long vacuum infiltration of tobacco plants. The hGM-CSF accumulation was found to be dependent on the presence of wetting agent supplemented during vacuum-agroinfiltration, duration of vacuum-agroinfiltration, and the age of the leaves. We demonstrate that industrial tobacco cultivars, with very high biomass, are suitable platforms for rapid, low cost production of foreign proteins. Successful transient expression of the GM-CSF in industrial tobacco was achieved in less than three months, opening the possibility for future applications of this approach in rapid response production of various proteins of non-plant origin.
Tobacco; transient expression; GM-CSF; vacuum infiltration; heterologous expression
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Podaci o prilogu
290-290.
2015.
objavljeno
Podaci o matičnoj publikaciji
Book of Abstracts of the Global Biotechnology Congress 2015
Boston (MA): Eureka Conferences Inc.
Podaci o skupu
Global Biotechnology Congress 2015
poster
22.07.2015-25.07.2015
Boston (MA), Sjedinjene Američke Države