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Rapid and low cost production of hGM-CSF cytokine in industrial tobacco – proof of concept (CROSBI ID 626901)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Vojta, Lea ; Ljuma-Skupnjak, Lana ; Budimir Ankica ; Vukičević Slobodan ; Fulgosi, Hrvoje Rapid and low cost production of hGM-CSF cytokine in industrial tobacco – proof of concept // Book of Abstracts of the Global Biotechnology Congress 2015. Boston (MA): Eureka Conferences Inc., 2015. str. 290-290

Podaci o odgovornosti

Vojta, Lea ; Ljuma-Skupnjak, Lana ; Budimir Ankica ; Vukičević Slobodan ; Fulgosi, Hrvoje

engleski

Rapid and low cost production of hGM-CSF cytokine in industrial tobacco – proof of concept

We report the production of hGM-CSF protein in leaves of two industrial cultivars of tobacco by using Agrobacterium-mediated transient expression. For overexpression, two binary destination vectors, p2GW7.0 and pH7WG2.0, were evaluated. The gene hGM-CSF was tagged on the 3’ end with the His and HA-epitopes and cloned into the destination vectors. Agrobacteria containing constructs GM-CSFHis- HA/pH7WG2.0 and GM-CSF-His-HA/p2GW7.0 were used for 5- and 10-minute-long vacuum infiltration of tobacco plants. The hGM-CSF accumulation was found to be dependent on the presence of wetting agent supplemented during vacuum-agroinfiltration, duration of vacuum-agroinfiltration, and the age of the leaves. We demonstrate that industrial tobacco cultivars, with very high biomass, are suitable platforms for rapid, low cost production of foreign proteins. Successful transient expression of the GM-CSF in industrial tobacco was achieved in less than three months, opening the possibility for future applications of this approach in rapid response production of various proteins of non-plant origin.

Tobacco; transient expression; GM-CSF; vacuum infiltration; heterologous expression

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Podaci o prilogu

290-290.

2015.

objavljeno

Podaci o matičnoj publikaciji

Book of Abstracts of the Global Biotechnology Congress 2015

Boston (MA): Eureka Conferences Inc.

Podaci o skupu

Global Biotechnology Congress 2015

poster

22.07.2015-25.07.2015

Boston (MA), Sjedinjene Američke Države

Povezanost rada

Biotehnologija, Biologija