engleski

Genetic parameters of 20 STR loci in the Croatian population

Allele frequencies and forensic parameters based on 20 short tandem repeat (STR) loci (D3S1358, vWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, TH01, FGA, D1S1656, D12S391, D10S1248, D22S1045, D2S441, D5S818, D13S317, D7S820, CSF1PO and TPOX) were determined. Buccal cell swabs of 400 unrelated individuals from Croatian population were collected. Genomic DNA was extracted by Chelex®-100 method and amplified using the AmpFlSTR®NGMTM and AmpFlSTR®Identifiler kits from Applied Biosystems - Life Technologies (AB) in a final reaction volume of 13 μL. Analysis of the amplified products was performed by capillary electrophoresis injection on the AB 3130xl Genetic Analyzer and the run data were analyzed using GeneMapper®ID software v.3.2 in accordance with the manufacturer’s recommendations. Statistical analysis for allele frequencies, power of discrimination, matching probability, power of exclusion, polymorphism information content and typical paternity index was performed using PowerStatsV12. The agreement of genotype frequencies with Hardy-Weinberg equilibrium was determined based on the number of observed and expected genotypes using Arlequin software v.3.5.1.2. The three loci, D1S1656, D12S391 and D18S51, had the highest power of discrimination (0.979, 0.974 and 0.970 respectively), as well as polymorphism information content (0.89 and 0.87, respectively). The results contribute to the existing Croatian DNA database as a useful tool for forensic identification and paternity testing.

STRs; AmpFlSTR Identifiler; AmpFl NGM; population data; Croatians

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